Ubiquitous Expression of bcl-2 Protein in Mitotic Nuclei and Chromosomes
スポンサーリンク
概要
- 論文の詳細を見る
We investigated the expression of bcl-2 oncoprotein in human cells by immunocytochemistry using an anti-bcl-2 antibody. Expression of bcl-2 was detected in and around mitotic nuclei and chromosomes from prophase to early telophase in all types of long-term cultured cell lines irrespective of the level of its expression in the cytoplasm. Western blot analysis showed a discrete but distinct protein band, which corresponded to the 26kDa α-form of bcl-2, in the lysate of cells showing only mitotic bcl-2. No other immunoreactions, including those corresponding to the β-form, were detected. RT-PCR combined with nested PCR showed bcl-2 mRNA in the same cell lines and in situ hybridization confirmed its presence in the cytoplasm of almost all interphase cells. The appearance of nuclear bcl-2 was not due to aberrant genetic regulation induced by an artificial in vitro environment. It was also not unique to neoplastic cells as nuclear and chromosomal bcl-2 was detected in freshly prepared specimens from B-cell lymphomas, myelodysplastic syndrome, and primary cultures of normal fibroblasts during mitosis. On the other hand, the level of cytoplasmic bcl-2 varied from one cell line to another; 8 carcinoma lines did not express cytoplasmic bcl-2, whereas many B cell lines expressed high levels of the protein. Bcl-2 appeared to accumulate in mitotic nuclei, because immunocytochemical reaction product was always stronger in mitotic nuclei than in the cytoplasm when the expression of bcl-2 was induced in peripheral blood lymphocytes by ConA stimulation. Our results indicate that bcl-2 protein is ubiquitously expressed in all cells around mitotic nuclei or chromosomes, whereas its distribution in the cytoplasm seems to reflect differences in genetic regulation based on cell lineage, differentiation stage or neoplastic state.
- 日本組織細胞化学会の論文
- 1998-12-01
著者
-
MORIKAWA Shigeru
Department of Molecular Biology and Medicine, Research Center for Advanced Science and Technology, U
-
Morikawa S
Department Of Pathology First Unit Shimane Medical University
-
Harada Takayuki
Second Unit Shimane Medical University
-
Harada Takayuki
Department Of Pathology Shimane Medical University
-
Harada Takayuki
Department Of Molecular Neurobiology Tokyo Metropolitan Institute For Neuroscience
-
Morikawa Shigeru
Department Of Pathology First Unit Shimane Medical University
-
Morikawa Shigeru
Department Of Pathology Shimane Medical University
-
Nakano Akinobu
Department Of Pathology Organ Pathology Unit Shimane University School Of Medicine
-
Morikawa Shigeru
Department Of Bacteriology And Department Of Pathology Chest Disease Research Institute Kyoto Univer
関連論文
- Effect of Electrical Stimulation on IGF-1 Transcription by L-Type Calcium Channels in Cultured Retinal Muller Cells
- The Effect of Statins on mRNA Levels of Genes Related to Inflammation, Coagulation, and Vascular Constriction in HUVEC
- 精製組換え核タンパク質を用いたELISAによるリンパ球性脈絡髄膜炎ウイルス標準株および分離株に対する抗体の検出
- Relative Induction of mRNA for HMG CoA Reductase and LDL Receptor by Five Different HMG-CoA Reductase Inhibitors in Cultured Human Cells
- Human leukocyte antigen-class II-negative long-term cultured human T-cell leukemia virus type-I-infected T-cell lines with progressed cytological properties significantly induce superantigen-dependent normal T-cell proliferation
- NUMBNESS OF THE RIGHT HAND AS THE INITIAL SYMPTOM IN CREUTZFELDT-JAKOB DISEASE
- Induced maturation of granules of a mouse mastocytoma cell line
- Cytochemical analysis of human leukemia-lymphoma cell lines. III. Staining pattern and localization of acid αnaphthyl acetate esterase activity.
- Two mastocytoma cell lines of NZB mice origin
- Detection of poly(ADP-ribose) in blood cells by immunofluorostaining method.
- Morphological characteristics of a human mammary carcinoma cell line (HPL-MmC)
- Thymectomy in Mice : Technical Improvement and Considerations of a Successful Surgical Procedure
- Enzyme Cytochemical Analysis of Long-Term Cultured Human Leukemia-Lymphoma Cell Lines (I)
- Studies on the Distribution and Immunohistochemical Localization of Xanthine Oxidase in Bovine Tissues
- Studies on Delayed Hypersensitivity in Mice II. T-Cell Dependency of the Response : T Cell; Limiting Cells in Induction of Delayed Footpad Reaction
- Immunohistological studies on lysozyme
- IMMUNOHISTOCHEMICAL STUDIES ON LACTATE DEHYDROGENASE SUBUNITS IN LUNG CANCER CELLS
- Immunohistological Studies on Xanthine Oxidase
- Immunohistochemical Studies on Lactate Dehydrogenase Subunits (II)
- Immunohistochemical Studies on Lactate Dehydrogenase Subunit (I)
- Intratubular germ cell neoplasia of unclassified type occupying the whole testis accompanied by a small mature teratoma and metastatic choriocarcinoma and Sertoli cell-only tubules in the other testis
- A new mutation, ataxia and male sterility (ams), of autoimmuneprone MRL/lpr mouse is not linked to lpr gene but associated with reduction of spleen size and alteration of lymphocyte subpopulations
- Brain stem glioblastoma with multiple large cyst formation and leptomeningeal dissemination in a 4-year-old girl
- Severe Optic Disc Edema Without Hydrocephalus in Neurofibromatosis 2
- Trochlear Nerve Palsy Associated With Superficial Siderosis of the Central Nervous System
- VISUAL FUNCTION IN PATIENTS WITH OPTIC NEURITIS ASSOCIATED WITH ACUTE TRANSVERSE MYELOPATHY IN MULTIPLE SCLEROSIS
- OPTIC NEUROPATHY AND ACUTE TRANSVERSE MYELOPATHY IN PRIMARY SJOGREN'S SYNDROME
- The distribution of endocrine cells in the surgically resected stomach
- Effects of Monensin and Tunicamycin on Cell Surface Expression of Carcinoembrionic Antigen in a Human Gastric Cancer Cell Line
- Function of the EGF-Receptor Expressed on Human Gastric Adenocarninoma Cell Lines.
- Analysis of Carcinoembryonic Antigen on Human Gastric Cancer Cell Lines by Flowcytometry.
- A human B-lineage dendritic cell line, HBM-Noda and its potential role in human T-cell leukemia/lymphoma virus type I infection
- Expression of NG2-Positive Cells During Optic Neuritis
- Expression of Epiplakin1 in the Developing and Adult Mouse Retina
- Inhibition of glial cell activation ameliorates the severity of experimental autoimmune encephalomyelitis
- IA-06 A mutant Ataxia and Male Sterility (AMS) mouse : 11. ams mutation causes accelerated cell death of the external granular layer of the retina induced by photic injury(THE 46TH ANNUAL MEETING OF THE JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY)
- Different types of neural cell death in the cerebellum of the ataxia and male sterility (AMS) mutant mouse
- Immunohistochemical Studies on the Distribution of Cytokeratin and Actin in Human Esophageal Cancer Cell Lines
- Investigation on the Expression of Epidermal Growth Factor-Receptor (EGF-R) of Human Gastric Adenocarcinoma Cells
- Cytological Characteristics of Four Longterm Cultured Human Gastric Adenocarcinoma Cell Lines
- Effect of TPA or retinoic acid on the production of carcinoembryonic antigen by human gastric carcinoma cell lines
- INHIBITORY ACTION OF PHA ON THE APPEARANCE OF THYMIC RETICULAR CELLS IN VITRO
- IMMUNOHISTOCHEMICAL STUDIES ON LACTATE DEHYDROGENASE SUBUNITS IN NORMAL BOVINE TISSUES
- Immunohistochemical Localization of Lactate Dehydrogenase Subunits in Porcine Liver; Effect of Fixatives on Detection of Specific Fluorescence
- No.42 Particular Affinity Observed in Gamma Globulin Fraction to the α-Cell Cytoplasm of Langerhans Islet
- A new monoclonal antibody (IE8) reactive with dendritically shaped cells in the human tonsil
- Cytochemical analysis of human leukemia-lymphoma cell lines. : V. Enzyme-cytochemical activities of B-cell lineage cell lines as markers of their differentiation : Relationship between acid phosphatase activity and cell functions.
- Enzyme Cytochemical Analysis of Long-term Cultured Human Leukemia-Lymphoma Cell Line : (IV) Enzyme Activities and Differentiation Markers in T-lineage Cell Lines.
- Diffuse Large B-cell Lymphoma in the Ampulla of Vater Causing Obstructive Jaundice : Report of a Case
- Enzyme-cytochemical analysis of long-term cultured human leukemia-lymphoma cell lines (II) Tartrate sensitive and resistant acid phosphatase activity
- P-14 Enzyme-and Immuno-histochemical Analysis of Human Hematopoietic Cell Lines with Endocytotic Activity
- Ubiquitous Expression of bcl-2 Protein in Mitotic Nuclei and Chromosomes
- 2. Immunohistochemistry of RNase (Enzyme Activity and Immunohistochemical Reaction)
- SPECIFICITY OF ANTI-LYMPHOCYTIC ANTIBODY AND ANTIGENICITY OF LYMPHOID CELLS
- In Vitro Antibiotic Susceptibility of Francisella tularensis Isolates from Japan
- Identification of the Source of Francisella tularensis Infection by Multiple-Locus Variable-Number Tandem Repeat Analysis