Immobilization of an Esterase Inhibitor on a Porous Hollow-Fiber Membrane by Radiation-Induced Graft Polymerization for Developing a Diagnostic Tool for Feline Kidney Diseases
スポンサーリンク
概要
- 論文の詳細を見る
Removal of the major urinary protein, cauxin, a carboxylesterase, from cat urine is essential for distinguishing between physiological and abnormal proteinuria by a urine dipstick. We have previously developed a material for removing cauxin by using lens culinaris agglutinin (LCA) lectin which targets the N-linked oligosaccharides present in cauxin. To improve the affinity and specificity toward cauxin, we immobilized 1,1,1-trifluoro-3-(2-sulfanylethylsulfanyl) propane-2-one, an inhibitor of esterases, to a polymer chain grafted on to a porous hollow-fiber membrane by applying radiation-induced graft polymerization. Normal male urine was forced to permeate through the pores rimmed by the ligand-immobilized polymer chain. Cauxin could not be detected in the effluent from the membrane. The residence time of the urine across a membrane thickness of 1 mm was set at 7 s. The respective dynamic and equilibrium binding capacities of the membrane for cauxin were 2 and 3 mg/g. The developed cauxin-affinity membrane material was more effective for diagnosing cat kidney diseases than the LCA lectin tip.
著者
-
Saito Kyoichi
Department Of Applied Chemistry And Biotechnology Chiba University
-
Matsuno Shinya
Department Of Anesthesiology Hirosaki National Hospital
-
Yamashita Tetsuro
Faculty Of Agriculture Iwate University
-
Umeno Daisuke
Department Of Applied Chemistry And Biotechnology Chiba University
-
SUZUTA Yasuyuki
Nippon Zenyaku Kogyo Co., Ltd.
-
MATSUNO Shinya
Department of Applied Chemistry and Biotechnology, Chiba University
-
MIYAZAKI Masao
Faculty of Agriculture, Iwate University
関連論文
- Fractional Elution and Determination of Uranium and Vanadium Adsorbed on Amidoxime Fiber from Seawater
- Affinity Precipitation Separation of DNA Binding Protein Using Block Conjugate Composed of Poly(N-isopropylacrylamide) Grafted Double-Stranded DNA and Double-Stranded DNA Containing a Target Sequence
- Modification of a Porous Sheet (MAPS) for the High-Performance Solid-Phase Extraction of Trace and Ultratrace Elements by Radiation-Induced Graft Polymerization
- Photodegradable Toners for Electrophotography III. Accelerated Photodegradation and Suppressed Photocrosslinking of Matrix Resin-Their Dependence on Polymer Composition, UV-light Source, and Irradiation Conditions
- Conductance Switching Phenomena and H-Like Aggregates in Squarylium-Dye Langmuir-Blodgett Films : Surfaces, Interfaces, and Films
- A Surface-Silylated Single-Layer Resist Using Chemical Amplification for Deep Ultraviolet Lithography:II. Limited Permeation of Si Compounds from Liquid Phase^* : Instrumentation, Measurement, and Fabrication Technology
- Photodegradable Toners for Electrophotography II. Accelerated Photodegradation of Matrix Resin by Deep Ultraviolet-Exposure at an Elevated Temperature
- A Surface-Silylated Single-Layer Resist Using Chemical Amplification for Deep-Ultraviolet Lithography : I.Limited Permeation of Si Compounds from Vapor Phase
- Enhancement of Dry-Etching Durabilities by Energy or Etchant Quenching with Aliphatic, Aromatic and Alicyclic Homopolymers, Polymer Blends and Copolymers
- Energy Quenching Effects on Dry-Etching Durability of Copolymers and Polymer Blends of Vinylnaphthalene or a-Methyl Styrene with Methyl Methacrylate
- Large Electric Conductance in the Interface Direction of Polar/Nonpolar Double-Layered Hetero-Langmuir-Blodgett Films
- Photodegradable Toners for Electrophotography I. Photodegradability of Matrix Resin
- A specific peptide produced during adult diapause of the leaf beetle, Gastrophysa atrocyanea Motschulsky (Coleoptera : Chrysomelidae)
- Synthesis and Characterization of Vinyl Polymer-Grafted DNA and Its AB-Type Block Conjugate with Native DNA Fragment
- High-performance purification of gelsolin from plasma using anion-exchange porous hollow-fiber membrane
- Evaluation of fluid volume status with a glucose challenge test in a patient with acute adrenal insufficiency
- Functional Mimicry of the Silkworm Diapause Hormone by an Insect Paralytic Peptide
- A Double Chaperone Function of the sHsp Genes against Heat-Based Environmental Adversity in the Soil-Dwelling Leaf Beetles
- Antheraea yamamai Paralytic Peptide Induces Egg Diapause as Well as Larval Paralysis in Bombyx mori : the Primary Sequence-Activity Correlations
- Immunoaffinity Purification and Identification of the Molecular Chaperone Calnexin
- Novel Propeptin Analog, Propeptin-2, Missing Two Amino Acid Residues from the Propeptin C-Terminus Loses Antibiotic Potency
- High-speed recovery of germanium in a convection-aided mode using functional porous hollow-fiber membranes
- Protein binding to polymer brush, based on ion-exchange, hydrophobic, and affinity interactions
- Calreticulin is Transiently Induced after Immunogen Treatment in the Fat Body of the Silkworm Bombyx mori
- Temperature - Induced Precipitation of Specific DNA Fragments Using DNA - Poly(N - isopropylacrylamide) Conjugate
- Removal of Cesium Using Cobalt-Ferrocyanide-Impregnated Polymer-Chain-Grafted Fibers
- Characteristics of the Stimulatory Effect of Kisspeptin-10 on the Secretion of Luteinizing Hormone, Follicle-Stimulating Hormone and Growth Hormone in Prepubertal Male and Female Cattle
- A history of aggression is a risk factor for postoperative confusion in elderly male drinkers
- Electrodialysis of Sulfuric Acid with Cation-Exchange Membranes Prepared by Electron-Beam-Induced Graft Polymerization
- Effects of leptin and leptin peptide amide on the release of luteinizing hormone, growth hormone and prolactin from cultured porcine anterior pituitary cells
- Mulberry stem bark extract stimulates in vitro immunomodulatory response in mouse spleen lymphocytes
- Poly(N-isopropylacrylamide) Carrying Double-Stranded DNA for Affinity Separation of Genotoxins
- Removal of Cesium Using Cobalt-Ferrocyanide-Impregnated Polymer-Chain-Grafted Fibers
- Sex steroid hormones do not enhance the direct stimulatory effect of kisspetin-10 on the secretion of growth hormone from bovine anterior pituitary cells
- A System for the Rapid Determination of the Mutation Spectrum in Escherichia coli
- Protein Resolution in Elution Chromatography Using Novel Cation-Exchange Polymer-Brush-Immobilized Particles
- Organic Bistable Memory Switching Phenomena in Squarylium-Dye Langmuir-Blodgett Films
- Energy Quenching Effects on Dry-Etching Durability of Alicyclic-Aliphatic Copolymers and Polymer Blends Enhanced by Polymer-Chain Entanglement
- 4Ap10 Evolutionary pathway engineering: toward the fast-track construction of long-step, but high-fidelity pathway for non-natural compounds
- Purification and Characterization of UDP-Arabinopyranose Mutase from Chlamydomonas reinhardtii
- Determination of Mole Percentages of Brush and Root of Polymer Chain Grafted onto Porous Sheet
- Immobilization of an Esterase Inhibitor on a Porous Hollow-Fiber Membrane by Radiation-Induced Graft Polymerization for Developing a Diagnostic Tool for Feline Kidney Diseases
- Adsorptive characteristics of fibrous adsorbent for uranium.
- Chemical forms of uranium in artificial seawater.