Accumulation of Xenotransplanted Canine Bone Marrow Cells in NOD/SCID/γcnull Mice with Acute Hepatitis Induced by CCl4

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概要

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• Bone marrow cell infusion (BMI) has recently been suggested as an effective therapy for refractory liver disease; however, the efficiency of BMI using canine bone marrow cells (cBMCs) has not been reported. We evaluated the accumulation potential of cBMCs in a mouse model of acute liver failure. Acute hepatitis was induced by carbon tetrachloride (CCl4) treatment in NOD/SCID/γcnull (NOG) mice and wild-type (WT) C57BL mice and the characteristics of liver dysfunction and the degree of hepatic injury and regeneration was compared between the two mouse models. Next, female CCl4-treated NOG mice were xenotransplanted with male PKH26-labeled cBMCs and the potential of cBMCs to accumulate in injured liver tissue compartments was examined. Fluorescence microscopy was performed to histologically detect the infused cBMCs and DNA polymerase chain reaction was performed for detection of the male Y chromosome (SRY gene) in the recipient female NOG mice. The number of PKH26-positive cBMCs transplanted in the liver tissue gradually increased in the NOG mice. The infused cBMCs were located in the necrotic area of the liver at an early stage after transplantation, and most had accumulated a week after transplantation. However, the therapeutic efficacy of the xenotransplantation remained unclear because no significant differences were observed an extent of the liver injury and regeneration between the cBMCs transplanted and saline control mice. These results suggest that cBMCs will specifically accumulate in injured liver tissue and that BMC transplant may have the potential to repair liver deficiency.

著者

• NEO Sakurako

  Laboratory of Internal Medicine II, School of Veterinary Medicine, Azabu University

• Kobayashi Ryosuke

  Research Institute of Biosciences, Azabu University

• Tsuchiya Ryo

  Laboratory Of Internal Medicine Ii School Of Veterinary Medicine Azabu University

• Hisasue Masaharu

  Laboratory Of Internal Medicine Ii School Of Veterinary Medicine Azabu University

• Yasuno Kyohei

  Research Institute Of Biosciences Azabu University

• SEGAWA Kazuhito

  Laboratory of Internal Medicine II, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuoku, Sagamihara, Kanagawa 252–5201, Japan

• YASUNO Kyohei

  Research Institute of Biosciences, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuoku, Sagamihara, Kanagawa 252–5201, Japan

• KATO Takashi

  Laboratory of Veterinary Internal Medicine II, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuoku, Sagamihara, Kanagawa 252–5201, Japan

• FUJIMOTO Ayumi

  Laboratory of Internal Medicine II, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuoku, Sagamihara, Kanagawa 252–5201, Japan

• MAKIISHI Eri

  Laboratory of Internal Medicine II, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuoku, Sagamihara, Kanagawa 252–5201, Japan

• KATO Takashi

  Laboratory of Internal Medicine II, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuoku, Sagamihara, Kanagawa 252–5201, Japan

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