Analysis of Valproate in Serum by Isotachophoresis

概要

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A simple, rapid and sensitive method for the determination of valproate in serum by isotachophores is (IP) has been established using a potential gradient detector and an isotachophoretic migration tube consisting of a pre-separation tube (6cm×1.0mm i. d.) and a separation capillary tube (15cm×0.5mm i. d.). The leading electrolyte was hydrochloric acid (5mM) adjusted to pH 4.3 with 6-amino-n-caproic acid. To the leading electrolyte was added 0.1% hydroxypropylmethylcellulose to reduce electroendosmosis. 2-(N-morpholino) ethanesulphonic acid served as the terminating electrolyte. Valproate was quantitativery separated without interference by such serum constituents as lactate and phosphate. Ten microliters of serum was injected directly into the apparatus. The analysis time was dramatically reduced by removing chloride ions from the serum sample in the pretreatment procedure with silver carbonate. The calibration curves (zone length-concentration plottings) for valproate in serum were found to be linear and to pass the origin in a concentration range of 1-300μg/ml. The present method was successfully applied to routine measurement of valproate quantity in serum and the results were found to agree fairly well with those obtained with GLC and HPLC.
Japan Society of Clinical Chemistryの論文