フェニルヒドラジン類を用いたマロンアルデヒドの高速液体クロマトグラフィーにおける誘導体化反応の比較検討(<特集>バイオアナリティカルケミストリー)

概要

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Malonaldehyde (MA) reacted with phenylhydrazines to form pyrazole derivatives. The behavior of the three hydrazines, 2, 4-dinitrophenyl- (DNPH), p-nitrophenyl- (NPH) and phenyl- (PhH), in the reaction with MA was compared by HPLC. A Shimadzu LC-5A HPLC equipped with a UV detector (315 nm for DNPH-MA and NPH-MA, 265 nm for PhH-MA) was used for the analysis. A metal column (25 cm × 4.6 mm i. d.) was packed with 5-μm Develosil ODS (Nomura Chemical Co., Ltd., Japan). Elution was carried out with CH_3CN-0.01 M HCl (40 : 60, v/v) at a flow rate of 1.5 ml/min. The reaction of MA with DNPH required a strongly acidic condition, while NPH and PhH reacted well with MA in a weakly acidic medium. The highest peak of the three derivatives was observed from reaction with NPH. The results above showed NPH to be quite useful for estimating a trace amount of free MA in a mild condition.
社団法人日本分析化学会の論文
1989-11-05