ガスクロマトグラフィーによるキョウニン水中のマンデルニトリルの定量

概要

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Apricot Kernel Water contains mandelonitrile and free benzaldehyde. A method for gas chromatographic(GC) determination of them is described. Procedure 1 : for determination of the total of both components, 0.25 ml of Apricot Kernel Water is diluted with water to 5 ml and 2 drops of 10% ammonia solution is added. The total of free benzaldehyde and one produced from mandelonitrile is extracted with 2 ml of ethyl acetate containing 2.0 mg of naphthalene as an internal standard saturating with sodium chloride. An aliquot of the extract is subjected to GC. GC conditions are as follows : 5% DEGS, 1.5m, 70℃, FID. Proccdure 2 : for determination of mandelonitrile, the same volume of sample solution is treated with 0.3 ml of 1 % hydroxylamine hydrochloride solution, which react with free benzaldehyde. After an excess of hydroxylamine is removed by adding 0.2 ml of acetone, the mixture is treated with 2 drops of 10% ammonia solution and the benzaldehyde produced corresponding to mandelonitrile is determined as directed in Procedure 1. This method was applied to analysis of a colored preparation composed of Apricot Kernel Water, Inf. Seneg. Rad. and Brocin (1:4:5), in which all mandelonitrile was found to have been decomposed to benzaldehyde.
社団法人日本分析化学会の論文
1979-03-05