細管式等速電気泳動法によるイタドリ中のシュウ酸分析

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Hot water extract from the stem of Polygonum cuspidatum, collected in April and May, was subjected to isotachophoresis in which a leading electrolyte containing 10 mm Cl^- as the leading ion and β-alanine as the counter ion (aqueous, pH 3.6), and a terminal electrolyte of 10 mm sodium n-hexanoate were used. The quantity of water-extractable oxalic acid in the stem varied greatly (184-8160 ppm) depending on the places and seasons those specimens were collected. The amount of soluble oxalic acid was shown to be small (184 ppm) in edible young stem, and to increase as the plant grows. Soluble oxalic acid extracted with boiling water for ten minutes from the stem however was found to be less than one tenth the quality of oxalic acid contained in the fresh stem. Subsequent extraction of the stem with boiling 2N HCl gave less soluble oxalate, which was much more in quantity than water-soluble oxalic acid. The ratio of the amount of oxalic acid to that of calcium ion in the 2N HCl extract showed that majority of the sparingly soluble oxalate in the stem is in the form of calcium salt. Other organic acids contained in the stem were found to be tartaric acid, malic acid, citric acid, and cinnamic acid. Rumex acetosa, Rhei Rhizoma, Polygoni Radix, Paeoniae Radix, Oxalis corniculata and spinach were also subjected to the analysis of oxalic acid.
1984-06-20