The Distribution of Human Pancreatic Elastase in Acute Pancreatitis Serum

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The distribution of human pancreatic elastase 2 in normal and pancreatitis serum was studied and a radioimmunoassay for determination of human pancreatic elastase 2 was developed. The distribution of human pancreatic elastase 2 was investigated by gel filtration on Sephadex G-200 column. The mixture of 125I-labeled elastase 2 (2μg) with normal serum (0.2ml) was subjected to gel filtration. Approximately 60% of elastase 2 formed a2 macroglobulin (α2M)- elastase 2 complex and remaining 40% formed al antitrypsin (α1AT)-elastase 2 complex. When the amount of elastase 2 was increased to 10μg, the amount of elastase 2 bound to a2M was approximately 30% and the amount of elastase 2 bound to al AT was 70%. In the case of acute pancreatitis serum (0.2ml), the distribution of elastase 2 was similar to that of 10μg elastase 2 mixed with normal serum. It was thought that when the elastase 2 increased in serum, excess elastase 2 formed complex more likely to α1 AT than to α2M. In the development of radioimmunoassay, radioiodinated tracer of elastase 2 was inactivated with phenylmethylsulfonyl fluoride (PMS), inhibitor of serine proteinase in order to prevent binding of elastase 2 to α2M and al AT in serum. α2M-elastase 2 complex showed no immunoreactivities, whereas al AT-elastase 2 complex was immunoreactive. Radioimmunoassayable elastase 2 in human serum was a part of α1 AT-elastase 2 complex. The minimum radioimmunoassayable concentration of elastase 2 was 8ng/ml. The average elastase 2 level in normal serum was 294ng/ml with a standard deviation of 82ng/ml. The concentration of serum elastase 2 in acute pancreatitis was elevated and the maximum amount was 4,200ng/ml. The concentration of serum elastase 2 in chronic pancreatitis was almost the same amount as normal serum.

The Distribution of Human Pancreatic Elastase in Acute Pancreatitis Serum

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