Selection of liquid chromatographic adsorbents for the selection and determination of uric acid in biological samples

概要

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Selection of liquid chromatographic adsorbents was studied for the separation and determination of uric acid in body fluids and mammalian tissues. A solution containing norepinephrine, epinephrine, dopamine, ascorbic acid and uric acid was used as a test solution for optimizing chromatographic conditions. Eluate from a column was electrochemically monitored by aid of an electrochemical detector set at +800mV <I>vs</I> Ag/Ag Cl. Five column systems were examined for the complete separation of catecholamines, ascorbic acid and uric acid. All the column systems examined in this study allowed the determination of uric acid in body fluids such as serum, cerebrospinal fluid and urine under the present chromatographic conditions. The reversedphase column systems examined in this study have been found to allow the quantitative determination of uric acid in mammalian tissues as well as in body fluids.
Japanese Society of Gout and Nucleic Acid Metabolismの論文