A Method for Determination of Endogeneous Uric Acid Levels in Biological Fluid by HPLC - ECD

概要

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A simple, easy, rapid, accurate, sensitive and selective method for the determination of endogeneous uric acid levels in body fluids such as serum, cerebrospinal fluid, and urine by reversed-phase high-performance liquid chromatography with electrochemical detection has been developed. In this article, the effectiveness of the chromatographic method is described showing some examples. The analytical recoveries of uric acid in body fluids have been found to be almost 100%. Complete separation of uric acid and ascorbic acid in body fluids was achieved in about 2 min by injecting a simply deproteinized body fluid sample onto the reversed-phase column under the present chromatographic conditions. The minimum detectable quantity of uric acid was found to be about 10 pg.
Japanese Society of Gout and Nucleic Acid Metabolismの論文