Antiproliferative and Proapoptotic Effects of Tocopherol and Tocol on Activated Hepatic Stellate Cells
スポンサーリンク
概要
- 論文の詳細を見る
Activated hepatic stellate cells (HSCs) play crucial roles in liver fibrosis. In the course of liver injury, HSCs, which reside in perisinusoidal spaces and lose lipid droplets, morphologically change into a myofibroblastic phenotype and acquire an increased proliferation activity in what is known as the activated state. We have investigated therapeutic strategies for liver fibrosis by promoting spontaneous reversion or inducing apoptosis in activated HSCs. Vitamin E consists of four tocopherols and four tocotrienols, all of which are well-known antioxidants. In this study, the antiproliferative and proapoptotic effects of a tocol, which lacks methyl groups attached to the chromanol ring, and four tocopherols were investigated using activated HSCs. δ-Tocopherol and tocol exhibited relatively high proliferation inhibitory and proapoptotic abilities. However, they did not show proliferation inhibition ability on primary hepatocytes or HepG2 cells. Significant cell detachment was also observed in δ-tocopherol- and tocol-treated HSCs. Decreased protein expressions of α-smooth muscle actin and β1 integrin were observed in a dose-dependent manner. These results indicate that δ-tocopherol and tocol induce anoikis in activated HSCs.
著者
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Imai Katsuyuki
Department Of Anatomy Akita University School Of Medicine
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MEZAKI Yoshihiro
Department of Cell Biology and Histology, Akita University School of Medicine
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Morii Mayako
Department Of Pediatric Surgery Akita University School Of Medicine
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Yamaguchi Noriko
Department Of Hygienic Sciences Kobe Pharmaceutical University
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Yoshikawa Kiwamu
Department Of Cell Biology And Histology Akita University School Of Medicine
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Miura Mitsutaka
Department Of Anatomy Akita University School Of Medicine
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HEBIGUCHI Tatsuzo
Department of Pediatric Surgery, Akita University Graduate School of Medicine
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MEZAKI Yoshihiro
Department of Cell Biology and Morphology, Akita University Graduate School of Medicine
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MIURA Mitsutaka
Department of Cell Biology and Morphology, Akita University Graduate School of Medicine
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IMAI Katsuyuki
Department of Cell Biology and Morphology, Akita University Graduate School of Medicine
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YOSHIKAWA Kiwamu
Department of Biochemistry, Akita University School of Medicine
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YAMAGUCHI Noriko
Department of Cell Biology and Histology, Akita University School of Medicine
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YAMAGUCHI Noriko
Department of Cell Biology and Morphology, Akita University Graduate School of Medicine
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