NONLYSOSOMAL GRANULES IN PERITONEAL MACROPHAGES : SUBCELLULAR LOCALIZATION OF NEUTRAL α-GLUCOSIDASE (<Symposium>Cytochemistry of Macrophage)
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概要
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Neutral α-glucosidase in mouse peritoneal macrophages was shown to be associated with granules distinct from lysosomes. When post-nuclear supernatants were centrifuged in a Percoll density gradient containing 20 units/ml of heparin and 10 mM Tris-HCl (pH 7.2), neutral α-glucosidase activity was clearly separated from β-glucuronidase activity. Activity peaks for alkaline phosphodiesterase, a plasma membrane enzyme, and for UDP-galactosyl transferase activity, a Golgi enzyme, were detected in different fractions. Release of the enzyme activities into extracellular medium depending on phagocytosis or pharmacological stimuli, was also different. These observations suggested that neutral α-glucosidase localize in nonlysosomal granules in macrophages.
- 日本組織細胞化学会の論文
著者
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Imai Katsuyuki
Department of Anatomy, Akita University School of Medicine
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TAKANO Yasuo
Department of Diagnostic Pathology, Graduate School of Medicine and Pharmaceutical Sciences
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Imai Katsuyuki
Department Of Anatomy Akita University School Of Medicine
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Imai Katsuyuki
Department Of Biochemistry Shimane Medical University
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FUJIMORI Ken
Department of Chemistry, University of Tsukuba
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Fujimori Ken
Department Of Anatomy School Of Medicine Faculty Of Pharmaceutical Sciences Tokushima University
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Takano Yasuo
Department Of Anatomy Kochi Medical School
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FUJIMORI KEN
Department of Anatomy, Tokushima University, School of Medicine
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TAKANO Yasuo
Department of Anatomy, Kochi Medical School
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