Isolation of Highly Purified Rat Cerebral Lysosomes Using Percoll Gradients with a Variety of Calcium Concentrations
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概要
- 論文の詳細を見る
We were able to isolate a lysosomal fraction from rat brain which had a higher degree of purity than in previous studies with good recovery. This has been made possible by using percoll gradients following the swelling of mitochondria in the presence of calcium which would eliminate contamination from small amounts of mitochondria. By using percoll density gradient centrifugation after the swelling of mitochondria in the presence of calcium, cerebral lysosomes were purified 312-fold with the recovery of approximately 22 %, which is the highest reported for any cerebral lysosomal preparation. The most effective procedure for the separation was achieved by using 1.25 mM calcium incubation with post nuclear fraction. As brain lysosomes may play a major role not only in degrading macromolecules but also in their transport to the deposition site, obtaining purified rat cerebral lysosomes represents an important step in the study of the generation of macromolecules which accumulate in the brain.
- 日本衛生学会の論文
著者
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SATO Atsushi
Department of Planning for Drug Development and Clinical Evaluation, Tohoku University Graduate Scho
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TAGUCHI Tetsuya
Department of Surgical Oncology, Osaka University Graduate School of Medicine
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TAKANO Yasuo
Department of Diagnostic Pathology, Graduate School of Medicine and Pharmaceutical Sciences
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Miyamoto Emi
Nutrition Science Department Sanyo Gakuen College
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Takano Yasuo
Department Of Anatomy Kochi Medical School
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Taguchi Tetsuya
Department Of Breast And Endocrine Surgery Osaka University Graduate School Of Medicine
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Taguchi Tetsuya
Department Of Environmental And Occupational Health Kochi Medical School
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AKIMARU Kunihiro
Department of Environmental and Occupational Health, Kochi Medical School
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YAMASAKI Mieko
Department of Nursing, Kochi Women's University
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RYU Shuhei
Department of Nursing, Kawasaki University Medical Welfare
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Yamasaki Mieko
Department Of Nursing Kochi Women's University
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Ryu Shuhei
Department Of Nursing Kawasaki University Medical Welfare
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Awata Shiro
Department Of Health Science Kochi Women's University
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Sato Atsushi
Department Of Applied Chemistry Chubu University
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Akimaru Kunihiro
Department Of Environmental And Occupational Health Kochi Medical School
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Taguchi Tetsuya
Department Of Biology And Function In Head And Neck Yokohama City University Graduate School Of Medicine
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