Kinetic Analysis of Endocytosis and Intracellular Fate of Liposomes in Single Macrophages.
スポンサーリンク
概要
- 論文の詳細を見る
Endocytosis and the intracellular fate of liposomes in single mouse peritoneal macrophages were examined kinetically by fluorescence microphotometry. Liposomes labeled with N- (7-nitro-2, 1, 3-benzoxadiazol-4-yl) phosphatidylethanolamine or containing 8-amino-naphthalene-1, 3, 6-trisulfonate were promptly incorporated into macrophages on incubation at 37°C, but fluorescence increase caused by hydrolysis of 4-methylumbelliferyl-β-D-glucoside encapsulated in the liposomes was observed after 30min of incubation. The fluorescences of calcein and 8-hydroxy-1, 3, 6-pyrenetrisulfonate (HPTS) in liposomes, which were respectively quenched statically due to high concentration and dynamically by a co-entrapped fluorescence quencher, p-xylene-bis-pyridinium bromide, also increased from 30min after the start of liposome incorporation, indicating that macrophages require this period for intracellular delivery of liposomes from the cell surface to lysosomes. Measurement of the intraendosomal pH change in a single macrophage at 37°C with liposomes containing a pH-sensitive fluorescent marker, HPTS, showed that the pH value decreased continuously to a constant value of 5.5 in 30-40min after endocytosis, and this decrease was reversed on addition of NH4Cl, suggesting that acidification of endosomes is not a stepwise reaction and is coupled with delivery of liposomes. These fluorescence microphotometric systems using liposomes containing different fluorescent dyes should be useful for kinetic analyses of the endocytosis and intracellular fate of liposomes in various phagocytes.
- 社団法人 日本生化学会の論文
著者
-
Imai Katsuyuki
Department Of Anatomy Akita University School Of Medicine
-
Shono Masayuki
School Of Medicine Univrsity Of Tokushima
-
Yoshimura Tetsuro
Institute For Enzyme Research Univeristy Of Tokushima
-
Shono Masayuki
School of Medicine, University of Tokushima
-
Hong Keelung
Cancer Research Institute, University of California
関連論文
- REGULATORY ROLE OF EXTRACELLULAR MATRIX COMPONENTS IN EXPRESSION OF MATRIX METALLOPROTELNASES IN CULTURED HEPATIC STELLATE CELLS
- REGULATION OF MORPHOLOGY AND GENE EXPRESSION BY THREE-DIMENTIONAL STRUCTURE OF TYPE I COLLAGEN FIBRILS IN CULTURED HEPATIC STELLATE CELLS
- Extracellular matrix-regulated p53 expression and nuclear localization in cultured Detroit 562 cells derived from pharyngeal carcinoma
- Structure and function of the Recombinant Fifth Domain of Human β_2-Glycoprotein I: Effects of Specific Cleavage between Lys77 and Thr78^1
- Role of the N- and C- Terminal Domains of Bovine β_2- Glycoprotein I in Its Interaction with Cardiolipin^1
- Nuclear Deviation in Hepatic Parenchymal Cells on Sinusoidal Surfaces in Arctic Animals
- THE INVOLVEMENT OF MATRIX MIETALLOPROTEINASES IN BASEMENT MEMBRANE INJURY IN ACUTE ALLERGIC AIRWAY INFLAMMATION
- Further application of a two-step heparin affinity chromatography method using divalent cations as eluents : Purification and identification of membrane-bound heparin binding proteins from the mitochondrial fraction of HL-60 cells
- NONLYSOSOMAL GRANULES IN PERITONEAL MACROPHAGES : SUBCELLULAR LOCALIZATION OF NEUTRAL α-GLUCOSIDASE (Cytochemistry of Macrophage)
- Hepatic Stellate Cells-From the Viewpoint of Retinoid Handling and Function of the Extracellular Matrix-
- PRENATAL DIFFERENTIATION OF MONOAMINE-CONTAINING NEURONS IN THE LOCUS COERULEUS OF THE RABBIT : A HISTOFLUORESCENCE STUDY
- Adhesion between Cells and Extracellular Matrix with Special Reference to Hepatic Stellate Cell Adhesion to Three-dimensional Collagen Fibers
- Kinectic Analysis of Endocytosis and Intracellular Fate of Liposomes in Single Macrophages
- MOLECULAR MECHANISM OF MEMBRANE FUSION INDUCED BY CLATHRIN
- Antiproliferative and Proapoptotic Effects of Tocopherol and Tocol on Activated Hepatic Stellate Cells
- 2-III-23 Uptake and storage of vitamin A as lipid droplets in the cytoplasm in cells in lamina propria mucosae of rat intestine
- Differential Increases in the Expression of Intermediate Filament Proteins and Concomitant Morphological Changes of Transdifferentiating Rat Hepatic Stellate Cells Observed In Vitro
- Kinetic Analysis of Endocytosis and Intracellular Fate of Liposomes in Single Macrophages.