Evaluation of Recombinant 28 kDa Outer Membrane Protein of <i>Brucella abortus</i> for the Clinical Diagnosis of Bovine Brucellosis in Korea

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概要

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• <i>Brucella</i> spp. are Gram-negative, facultative, intracellular coccobacilli that are pathogenic to a variety of mammals, including ruminants and humans. The conventional serological test for diagnosing brucellosis in cattle in Korea is the standard tube agglutination test. However, agglutination tests sometimes give false-positive results due to cross-reactions with other pathogens. The outer membrane proteins of <i>Brucella</i> species have been extensively studied for their immunogenicity and serodiagnostic applications. However, an application of <i>B. abortus</i> OMPs for serodiagnosis has not been successfully established. In this study, cloning and expression of <i>B. abortus</i> Omp28, a group 3 antigen, were accomplished by PCR amplification cloning into a pMAL expression system, and purification of a recombinant Omp28 (rOmp28). The immunogenicity of rOmp28 was confirmed by Western blot analysis with <i>Brucella</i>-positive bovine serum. To determine whether rOmp2 has a potential benefit for use in the serodiagnosis of bovine brucellosis, rOmp28-based ELISA and latex bead agglutination test were performed. <i>B. abortus</i> positive (n=122) or negative (n=88) from TAT were positive (118/122, 96.7%) or negative (84/88, 95.4%) in ELISA and were positive (94/122, 77%) or negative (71/88, 81.7%) in that the latex bead agglutination test, respectively. The sensitivity, specificity and accuracy were 96.7, 95.4, 96.2% in ELISA and 77, 80.6, 78.5% in latex bead agglutination test, respectively. These findings suggest that the rOmp28 of <i>B. abortus</i> might be a good candidate for developing serological diagnostic tools for bovine brucellosis.

著者

• MIN Wongi

  College of Veterinary Medicine & Research Institute of Life Science, Gyeongsang National University

• Kim Dong

  College Of Pharmacy Kyung Hee University

• LEE Jin

  College of Oriental Medicine, Kyung Hee University

• CHANG Hong

  Institute of Agriculture and Life Science, Gyeongsang National University

• Rhee Man

  College Of Veterinary Medicine Kyungpook National University

• LIM Jeong

  College of Oriental Medicine, Kyunghee University

• Kim Dae

  College Of Life Sciences And Biotechnology Korea University

• KIM Suk

  College of Veterinary Medicine & Research Institute of Life Science, Gyeongsang National University

• LEE Hu

  College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea

• RHEE Man

  College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea

• LEE Jin

  College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea

• MIN Wongi

  College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, Republic of Korea

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