人血中β-lipotropin (β-LPH) の検討-その特異的ラジオイムノアッセイと血中動態

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A specific radioimmunoassay for human β-lipotropin (hβ-LPH) was developed employing the N-terminal antibody given by Dr. Choh Hao Li. At a final dilution of 1 : 18,000-24,000, the antiserum showed less than 0.28% crossreactivity with sheep β-LPH, human (h) - β-MSH, hGH and hPRL but none with h1-39 ACTH, hβ-endorphin, hLH, hFSH, hTSH and sheep β-endorphin. β-LPH was extracted from 2 ml plasma samples utilizing a 90 mg Vycor silica glass. Standard hβ-LPH was added to β-LPH free plasma and extracted along with the samples. Dried extracts were resuspended with a 300μl assay buffer, and duplicate 100 μl samples were used for radioimmunoassay. One hundred μl sample or standard, 100 μl buffer, 50 μl antiserum and 50 μl 125 I-hβ-LPH were incubated at 4°C for 3 days. Separation of bound and free fractions was achieved by second antibody precipitations. Immunoreactive β-LPH was much more stable in plasma than immunoreactive ACTH, and no loss of activity was found at 24 hr after the addition of hβ-LPH to plasma at room temperature. The lowest detectable level of plasma β-LPH was 30 pg/ml. The intra-assay coefficient of variation (3 assays) averaged 10.1%. Mean plasma β-LPH levels were 80.6 ± 29.1 pg/ml in 17 normal persons. Plasma β-LPH levels rose after metyrapone administration and were suppressed after dexamethasone. In patients with various diseases of the pituitary-adrenal axis, levels of β-LPH were parallel to the levels of immunoreactive ACTH. However, in patients with renal insufficiency, the levels of β-LPH were abnormally elevated both before and after the hemodialysis while plasma ACTH levels were normal.
日本内分泌学会の論文

人血中β-lipotropin (β-LPH) の検討-その特異的ラジオイムノアッセイと血中動態

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