イヌ胃体部粘膜におけるガストリンの作用機序に関する研究 (第2報):Ca<SUP>2+</SUP>-activated, phospholipid -dependent protein kinase 活性とリン脂質代謝におよぼすガストリンの影響
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The existence of Ca<SUP>2+</SUP>-activated, phospholipid-dependent protein kinase (protein kinase C), the effect of gastrin on phospholipid metabolism and guanylate cyclase activity were investigated to elucidate the cellular mechanism of action of gastrin on the corporal mucosa of the canine stomach.<BR>Protein kinase activity was determined by measuring the incorporation of [<SUP>32</SUP>P] into calf thymus H1-histone from [<SUP>32</SUP>] P-ATP. One unit of protein kinase was defined as the amount of enzyme which incorporated 1 pmol of phosphate from ATP into H<SUB>1</SUB>-histone. Protein kinase C was found in 100,000xg supernatant of homogenate fractionated by a DEAE-cellulose column chromatography. Characteristics of further purified protein kinase C, such as dependency on divalent cations and phospholipids, were in agreement with those of previously reported protein kinase C in other tissues. Furthermore, the gastric corporal mucosa was found to contain protein kinase C in large quantities. The specific activity of protein kinase C was 26,000 units/mg protein.<BR>The phospholipid metabolism was evaluated by the incorporation of [<SUP>14</SUP>C] -glycerol3-phosphate and the change of the radioactivity of [<SUP>32</SUP>P] in individual phospholipids. Each phospholipid was extracted from the gastric corporal mucosa and isolated by thin layer chromatography. Guanylate cyclase activity was determined by measuring the cGMP produced, using radioimmunoassay.<BR>Gastrin significantly increased the incorporation of [<SUP>14</SUP>C] -glycerol-3-phosphate into phosphatidylethanolamine in the presence of acethylcholine (Ach). Ach increased the uptake of the tracer into phosphatidylinositol significantly, and the increase was enhanced by the simultaneous addition of gastrin. In the experiments with [<SUP>32</SUP>] -labeled phospholipids, gastrin increased the incorporation of [<SUP>32</SUP>] into phosphatidylethanolamine significantly. The significant increase of the radioactivity in phosphatidylinositol by Ach failed to be enhanced by gastrin, but that of phosphatidylethanolamine by Ach was enhanced by gastrin.<BR>No stimulation of guanylate cyclase activity by gastrin was detected in the dispersed gastric corporal mucosal cells.<BR>These results indicate that gastric corporal mucosa was one of the most abundant tissues in which protein kinase C was contained, when compared with various mammalian tissues previously reported by Minakuchi, Nishizuka, et al. Nishizuka et al, recently proposed the novel hypothesis that phosphatidylisositol turnover activated by cAMP-independent agonists will be essentially required to activate protein kinase C. Our results suggest that gastrin can provoke phospholipids turnover including phosphatidylinositol turnover in gastric corporal mucosa.<BR>Therefore, our data indicate the possibility that the protein kinase C system plays an important role in the cellular mechanism of action of gastrin on gastric corporal mucosa.
- 日本内分泌学会の論文
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