125I-protein Aを用いた抗ヒトthyroglobulin抗体の固相法radioimmunoassayの検討

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A solid phase radioimmunoassay for the detection of anti-human thyroglobulin antibodies using 125I-protein A has been developed and its clinical use was evaluated in 20 patients with Graves' disease and 20 patients with Hashimoto's thyroiditis. After coating polystylene tubes with human thyroglobuline (10-2mg/ml), 50μl of sera were incubated for 2 hours at 25°C followed by the addition of 125I-protein A. After incubation, the tubes were washed extensively to remove free125I-protein A, and radioactivities of the bound 125I-protein A were counted. The results of this solid phase radioimmunoassay were compared with those obtained by two other commercially available kits : Thyroid test and Thyroglobulin antibody kit (125I-HTg method). Based on the results of the 125I-HTg and 125I-protein A method, obtained from the sera of 26 healthy subjects, normal range of P/N1 of the 125I.-HTg method (P : patient, N1 : anti-HTg antibody free serum) and P/N2 of the 125I-protein A method (P : patient, N2 : mean of healthy subjects) were obtained. P/N1 higher than 0.92 and P/N2higher than 1.64 were considered to be positive for anti-thyroglobulin antibodies. In Graves' disease, 7 out of 20 patients (35%) were positive by thyroid test. Positivity increased up to 10 (50%) by the 125I-HTg method, and 13 (65%) by the 125I-protein A method. Among 20 patients with Hashimoto's thyroiditis, 13 (65%) were positive by thyroid test, 17 (85%) were positive by the 125I-HTg method, and 20 (100%) were positive by the 125I-protein A method. Thus, sensitivity for the detection of anti-thyroglobulin antibodies by solid phase radioimmunoassay using125i-protein A was much higher than those obtained by commercially available kits. The whole procedure of this method is easy to operate as well. In conclusion, this solid phase radioimmunoassay using125I-protein A is useful for the detection of anti-thyroglobulin antibodies.
日本内分泌学会の論文

<SUP>125</SUP>I-protein Aを用いた抗ヒトthyroglobulin抗体の固相法radioimmunoassayの検討

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