Interleukin-6 Enhances Porcine Parthenote Development In Vitro, through the IL-6/Stat3 Signaling Pathway
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概要
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Signal transducer and activator of transcription-3 (Stat3) plays a central role in interleukin-6 (IL-6)-mediated cell proliferation by inhibiting apoptosis in a variety of cell types. The Stat3 pathway is essential for embryonic development. The aim of this study was to determine the effects of recombinant IL-6 on the viability and development of porcine diploid parthenotes cultured in vitro. Four-cell parthenotes, derived in vitro, were cultured to the blastocyst stage, with or without recombinant IL-6. The addition of 10 or 100 ng/ml of recombinant swine IL-6 into PZM3 medium increased the development rate of parthenotes to the blastocyst stage (P<0.05). When supplemented with 10 ng/ml of recombinant swine IL-6, the number of parthenotes at the blastocyst stage increased (P<0.05) and apoptosis decreased (P<0.05). Real-time RT-PCR experiments revealed that the addition of recombinant swine IL-6 decreased the mRNA expression of the pro-apoptotic gene Caspase3 (P<0.01) but increased the expression levels of the anti-apoptotic genes Bcl2l1 and Survivin. IL-6 receptors and Stat3 mRNA expression were upregulated after treatment with 10 ng/ml recombinant swine IL-6. Immunoblots and fluorescence labeling experiments showed that the levels of phosphorylated Stat3 were upregulated. These results suggest that recombinant swine IL-6 prevents apoptosis of porcine parthenotes and enhances porcine embryo viability through the IL-6/Stat3 signaling pathway in vitro.
著者
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Shen Xing-hui
Department Of Animal Sciences Chungbuk National University
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Cui Xiang-shun
Department Of Animal Sciences Chungbuk National University
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Kim Nam-hyung
Department Of Animal Sciences Chungbuk National University
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Lee Sung-hyun
Department Of Animal Sciences Chungbuk National University
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CUI Xiang-Shun
Department of Animal Sciences, Chungbuk National University, Chungbuk 361-763, Korea
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SHEN Xing-Hui
Department of Histology and Embryology, Harbin Medical University, Heilongjiang Province, China
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KIM Nam-Hyung
Department of Animal Sciences, Chungbuk National University, Chungbuk 361-763, Korea
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