Mitochondrial Dysfunction Influences Apoptosis and Autophagy in Porcine Parthenotes Developing In Vitro
スポンサーリンク
概要
- 論文の詳細を見る
Mitochondria are important regulators of both apoptosis and autophagy. One of the triggers for mitochondrial-mediated apoptosis is the production of reactive oxygen species (ROS), which include hydrogen peroxide, superoxide, hydroxyl radical, nitric oxide and peroxynitrite. Recently, several studies have indicated that ROS may also be involved in the induction of autophagy. In the present study, we used H2O2 to induce mitochondrial stress, examined apoptotic- and autophagic-related gene expression and observed LC3 protein (autophagosome presence marker) expression in porcine parthenotes developing in vitro. In porcine four-cell parthenotes cultured for 5 days in NCSU37 medium containing 0.4% BSA, the developmental rate and mitochondrial distribution did not differ from that of the group supplemented with 100 μM H2O2 but was significantly decreased in the group supplemented with 500 μM H2O2 (P<0.05). Transmission electron microscopy (TEM) indicated that whereas normal shaped mitochondria were observed in blastocysts from the control group, abnormal mitochondria (mitophagy) and autophagic vacuoles were observed in blastocysts from the group that received 500 μM H2O2. Furthermore, addition of H2O2 (100 μM and 500 μM) decreased cell numbers (P<0.05) and increased both apoptosis (P<0.05) and LC3 protein expression in the blastocysts. Real-time RT-PCR showed that H2O2 significantly decreased mRNA expression of anti-apoptotic gene Bcl-xL but increased pro-apoptotic genes, Caspase 3 (Casp3) and Bak, and autophagy-related genes, microtubule-associated protein 1 light chain 3 (Map1lc3b) and lysosomal-associated membrane protein 2 (Lamp2). However, the addition of H2O2 had no effect on mRNA expression levels in nuclear DNA-encoded mitochondrial-related genes, cytochrome oxidase (Cox) 5a, Cox5b and Cox6b1, in blastocysts. These results suggest that H2O2 leads to mitochondrial dysfunction that results in apoptosis and autophagy, which is possibly related to porcine early embryo development.
- 2011-02-01
著者
-
HWANG Kyu-Chan
Department of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University
-
KIM Nam-Hyung
Department of Animal Sciences, Chungbuk National University
-
Hwang Kyu-chan
Department Of Animal Biotechnology College Of Animal Bioscience And Technology Konkuk University
-
CUI Xiang-Shun
Department of Animal Sciences, Chungbuk National University
-
Cui Xiang-shun
Department Of Animal Sciences Chungbuk National University
-
Kim Nam-hyung
Department Of Animal Sciences Chungbuk National University
-
Lee Sung-hyun
Department Of Electronics Engineering Sejong University
-
Li Ying-hua
Department Of Animal Sciences Chungbuk National University
-
Xu Yong-nan
Department Of Animal Sciences Chungbuk National University
-
SUN Shao-Chen
Department of Animal Sciences, Chungbuk National University
-
LEE Seung-Eun
Department of Animal Sciences, Chungbuk National University
-
KWON Jung-Suk
Department of Animal Sciences, Chungbuk National University
-
Sun Shao-chen
Department Of Animal Sciences Chungbuk National University
-
Hwang Kyu-chan
Department Of Animal Sciences Chungbuk National University
-
Lee Seung-eun
Department Of Animal Sciences Chungbuk National University
-
Kwon Jung-suk
Department Of Animal Sciences Chungbuk National University
-
Lee Sung-hyun
Department Of Animal Sciences Chungbuk National University
関連論文
- Trichostatin A Promotes the Development of Bovine Somatic Cell Nuclear Transfer Embryos
- Production of Transgenic Pigs Harboring the Human Erythropoietin (hEPO) Gene Using Somatic Cell Nuclear Transfer
- Effect of Vanadate on the Chromatin Configuration in Pig GV-oocytes
- Hyperglycemia Reduces Mitochondrial Content and Glucose Transporter Expression in Mouse Embryos Developing In Vitro
- Maternal Gene Transcription in Mouse Oocytes: Genes Implicated in Oocyte Maturation and Fertilization
- Amorphous Silicon Film Deposition by Low Temperature Catalytic Chemical Vapor Deposition (
- Maternal Gene Transcription in Mouse Oocytes : Genes Implicated in Oocyte Maturation and Fertilization
- Low Salt Maturation Medium Enhances the Histone H1 Kinase Activity of Porcine Oocytes at the End of In Vitro Maturation
- Mitochondrial Dysfunction Influences Apoptosis and Autophagy in Porcine Parthenotes Developing In Vitro
- Regulation of Maternal Gene Expression by MEK/MAPK and MPF Signaling in Porcine Oocytes During In Vitro Meiotic Maturation
- Trichostatin A Promotes the Development of Bovine Somatic Cell Nuclear Transfer Embryos
- MicroRNAs in fertilized and cloned mouse embryos
- Chitosan as Morphology-directing Agent for the Preparation of Multiarmed Selenium/Carbon Coaxial Nanorods
- Electrical Activation Enhances Pre-Implantation Embryo Development Following Sperm Injection into In Vitro Matured Pig Oocytes
- Overtly Manifested Diabetes Mellitus after Resection of Insulinoma
- Autophagy Influences Maternal mRNA Degradation and Apoptosis in Porcine Parthenotes Developing In Vitro
- Interleukin-6 Enhances Porcine Parthenote Development In Vitro, through the IL-6/Stat3 Signaling Pathway
- Altered Gene Expression Profiles in Mouse Tetraploid Blastocysts
- Interleukin-6 Enhances Porcine Parthenote Development In Vitro, through the lL-6/Stat3 Signaling Pathway
- Autophagy Influences Maternal mRNA Degradation and Apoptosis in Porcine Parthenotes Developing In Vitro
- Knockdown of Maternal Homeobox Transcription Factor SEBOX Gene Impaired Early Embryonic Development in Porcine Parthenotes
- E2-9 Obstructive sleep apnea is associated with reduced quality of life in adult asthmatics(English Session 2)
- E2-6 Chronic cough in the elderly population : its impact on quality of life and the association with upper airway symptoms(English Session 2)
- Erratum: "Amorphous Silicon Film Deposition by Low Temperature Catalytic Chemical Vapor Deposition ($
- Production of Transgenic Pigs Harboring the Human Erythropoietin (hEPO) Gene Using Somatic Cell Nuclear Transfer