Regulation of Maternal Gene Expression by MEK/MAPK and MPF Signaling in Porcine Oocytes During In Vitro Meiotic Maturation
スポンサーリンク
概要
- 論文の詳細を見る
Mitogen-activated protein kinase (MAPK) and maturation/M phase promoting factor (MPF) play crucial roles in oocyte meiotic maturation in mammals. However, the underlying molecular mechanisms have not been addressed. In this study, the effects of the MEK/MAPK pathway inhibitor U0126 and the MPF inhibitor roscovitine on meiotic maturation and maternal gene expression in pig cumulus-oocyte complexes (COCs) and denuded oocytes (DOs) were investigated. Both inhibitors can reversibly block the resumption of meiosis in pig oocytes. COCs or DOs initially cultured in drug-free medium for 24 h and then transferred to medium containing U0126 showed normal progress to the Metaphase II stage (MII); (90.38 vs. 92.16% control group). In contrast, roscovitine treatment from 24 to 44 h significantly inhibited maturation of COCs and DOs. To explore the underlying molecular mechanisms, expression patterns and polyadenylation states of five representative maternal transcripts, cyclin B1, Cdc2, C-mos, GDF9 and BMP15, were examined by real-time PCR and poly(A)-test PCR (PAT assay). U0126 treatment resulted in aberrant expression of Cdc2 and GDF9, while roscovitine significantly maintained all five maternal transcripts at very high levels in treated COCs compared with the control group. The polyadenylation of these mRNAs increased as well. Furthermore, the experiments were repeated in DOs, and the results also indicated that both Cdc2 and GDF9 showed significantly higher expression in both mRNA and polyadenylation levels in the drug treatment groups. Together, these results provide the first demonstration in a mammalian system that MAPK and MPF play important roles in regulation of maternal gene expression during oocyte maturation.
- 2011-02-01
著者
-
KIM Nam-Hyung
Department of Animal Sciences, Chungbuk National University
-
CUI Xiang-Shun
Department of Animal Sciences, Chungbuk National University
-
Cui Xiang-shun
Department Of Animal Sciences Chungbuk National University
-
Kim Nam-hyung
Department Of Animal Sciences Chungbuk National University
-
Li Ying-hua
Department Of Animal Sciences Chungbuk National University
-
Xu Yong-nan
Department Of Animal Sciences Chungbuk National University
-
SUN Shao-Chen
Department of Animal Sciences, Chungbuk National University
-
ZHANG Ding-Xiao
Department of Animal Sciences, Chungbuk National University
-
PARK Won-Jun
Department of Animal Sciences, Chungbuk National University
-
Sun Shao-chen
Department Of Animal Sciences Chungbuk National University
-
Zhang Ding-xiao
Department Of Animal Sciences Chungbuk National University
-
Park Won-jun
Department Of Animal Sciences Chungbuk National University
関連論文
- Trichostatin A Promotes the Development of Bovine Somatic Cell Nuclear Transfer Embryos
- Hyperglycemia Reduces Mitochondrial Content and Glucose Transporter Expression in Mouse Embryos Developing In Vitro
- Maternal Gene Transcription in Mouse Oocytes: Genes Implicated in Oocyte Maturation and Fertilization
- Maternal Gene Transcription in Mouse Oocytes : Genes Implicated in Oocyte Maturation and Fertilization
- Low Salt Maturation Medium Enhances the Histone H1 Kinase Activity of Porcine Oocytes at the End of In Vitro Maturation
- Mitochondrial Dysfunction Influences Apoptosis and Autophagy in Porcine Parthenotes Developing In Vitro
- Regulation of Maternal Gene Expression by MEK/MAPK and MPF Signaling in Porcine Oocytes During In Vitro Meiotic Maturation
- Trichostatin A Promotes the Development of Bovine Somatic Cell Nuclear Transfer Embryos
- MicroRNAs in fertilized and cloned mouse embryos
- Chitosan as Morphology-directing Agent for the Preparation of Multiarmed Selenium/Carbon Coaxial Nanorods
- Autophagy Influences Maternal mRNA Degradation and Apoptosis in Porcine Parthenotes Developing In Vitro
- Interleukin-6 Enhances Porcine Parthenote Development In Vitro, through the IL-6/Stat3 Signaling Pathway
- Interleukin-6 Enhances Porcine Parthenote Development In Vitro, through the lL-6/Stat3 Signaling Pathway
- Autophagy Influences Maternal mRNA Degradation and Apoptosis in Porcine Parthenotes Developing In Vitro
- Knockdown of Maternal Homeobox Transcription Factor SEBOX Gene Impaired Early Embryonic Development in Porcine Parthenotes