Knockdown of Maternal Homeobox Transcription Factor SEBOX Gene Impaired Early Embryonic Development in Porcine Parthenotes
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概要
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A number of germ cell-specific transcription factors essential for ovarian formation and folliculogenesis have been identified and studied. However, the role of these factors during early embryonic development has been poorly explored. In the present study, we investigated the role of SEBOX, a maternal homeobox transcription factor, during early embryonic development in porcine parthenotes. mRNA for SEBOX is preferentially expressed in oocytes, and expression persists until embryonic genome activation (EGA). Knockdown of SEBOX by siRNA disrupted early embryonic development, but not oocyte maturation. Many maternal genes essential for early embryonic development were upregulated in SEBOX-depleted embryos. Moreover, some pluripotency-associated genes, including SOX2 and NANOG, were upregulated when SEBOX was knocked down. Therefore, our data demonstrate that SEBOX is required for early embryonic development in pigs and appears to regulate the degradation of maternal transcripts and the expression of pluripotency genes.
著者
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Cui Xiang-shun
Department Of Animal Sciences Chungbuk National University
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Zhao Ming-hui
Department Of Ophthalmology Shanghai Jiaotong University Affiliated Sixth People's Hospital
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Kim Nam-hyung
Department Of Animal Sciences Chungbuk National University
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Zheng Zhong
Department Of Biological Sciences And Biotechnology Tsinghua University
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Oh Jeong
Department Of Chemistry Kookmin University
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HEO Young-Tae
Department of Animal Sciences, Chungbuk National University
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JIA Jia-Lin
Department of Animal Sciences, Chungbuk National University, Cheongju 361-763, Korea
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KIM Nam-Hyung
Department of Animal Sciences, Chungbuk National University, Cheongju 361-763, Korea
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ZHAO Ming-Hui
Department of Animal Sciences, Chungbuk National University, Cheongju 361-763, Korea
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ZHENG Zhong
Department of Animal Sciences, Chungbuk National University, Cheongju 361-763, Korea
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CUI Xiang-Shun
Department of Animal Sciences, Chungbuk National University, Cheongju 361-763, Korea
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HEO Young-Tae
Department of Animal Sciences, Chungbuk National University, Cheongju 361-763, Korea
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