Low Salt Maturation Medium Enhances the Histone H1 Kinase Activity of Porcine Oocytes at the End of In Vitro Maturation
スポンサーリンク
概要
- 論文の詳細を見る
The effect of NaCl in maturation media on histone H1 kinase activity of porcine oocytes was examined at the end of culture for maturation. Oocyte-cumulus complexes were cultured in modified Whittens medium containing different concentrations of NaCl (44.50, 68.49, 92.40, 116.40 or 140.35 mM). The media were supplemented with 10% porcine follicular fluid and hormones for 20 h and then without hormonal supplements for an additional 20 h. At the end of culture, oocytes were sampled for morphological observation, for glutathione assay and for histone H1 kinase assay. At the end of culture, the incidence of oocytes at each meiotic stage was not different among groups. However, histone H1 kinase activity and glutathione content were higher (P<0.01) in oocytes cultured in media with lower NaCl concentrations. Histone H1 kinase activity in the oocytes at the end of maturation culture was correlated with intracellular glutathione content (r=0.899, P<0.01). These data indicate that histone H1 kinase activity and glutathione content of porcine oocytes at the end of culture for maturation are reduced when a high salt medium is used for maturation.
- 日本繁殖生物学会の論文
- 1996-05-01
著者
-
FUNAHASHI Hiroaki
Department of Animal Science, Graduate School of Natural Science and Technology, Okayama University
-
KIM Nam-Hyung
Department of Animal Sciences, Chungbuk National University
-
Funahashi Hiroaki
Department Of Animal Science Graduate School Of Natural Science And Technology Okayama University
-
Kim Nam-hyung
Department Of Animal Sciences Chungbuk National University
-
Day B
Department Of Animal Sciences University Of Missouri-columbia
-
Kim Nam-hyung
Department Of Animal Sciences University Of Missouri-columbia
-
Stumpf Todd
Department Of Animal Sciences University Of Missouri-columbia
-
DAY Billy
Department of Animal Sciences, University of Missouri-Columbia
-
Day Billy
Department Of Animal Sciences University Of Missouri-columbia
-
Funahashi Hiroaki
Department Of Animal Sciences University Of Missouri-columbia
-
Funahashi Hiroaki
Department Of Animal Science Faculty Of Agriculture Okayama University
関連論文
- Trichostatin A Promotes the Development of Bovine Somatic Cell Nuclear Transfer Embryos
- In-vitro Culture with a Tilting Device in Chemically Defined Media During Meiotic Maturation and Early Development Improves the Quality of Blastocysts Derived from In-vitro Matured and Fertilized Porcine Oocytes
- Successful Piglet Production in a Chemically Defined System for In-vitro Production of Porcine Embryos : Dibutyryl Cyclic AMP and Epidermal Growth Factor-family Peptides Support In-vitro Maturation of Oocytes in the Absence of Gonadotropins
- Hyperglycemia Reduces Mitochondrial Content and Glucose Transporter Expression in Mouse Embryos Developing In Vitro
- Hydrophobic Silicone Elastomer Chamber for Recording Trajectories of Motile Porcine Sperms without Adsorption
- Low Salt Maturation Medium Enhances the Histone H1 Kinase Activity of Porcine Oocytes at the End of In Vitro Maturation
- The Presence of Tissue Inhibitor of Matrix Metalloproteinase-1 (TIMP-1) During Meiosis Improves Porcine 'Oocyte Competence' as Determined by Early Embryonic Development After In-vitro Fertilization
- Mitochondrial Dysfunction Influences Apoptosis and Autophagy in Porcine Parthenotes Developing In Vitro
- Regulation of Maternal Gene Expression by MEK/MAPK and MPF Signaling in Porcine Oocytes During In Vitro Meiotic Maturation
- Hydrophobic Silicone Elastomer Chamber for Recording Trajectories of Motile Porcine Sperms without Adsorption
- Trichostatin A Promotes the Development of Bovine Somatic Cell Nuclear Transfer Embryos
- MicroRNAs in fertilized and cloned mouse embryos
- In-vitro Culture with a Tilting Device in Chemically Defined Media During Meiotic Maturation and Early Development Improves the Quality of Blastocysts Derived from In-vitro Matured and Fertilized Porcine Oocytes
- Exogenous Adenosine Reduces the Mitochondrial Membrane Potential of Murine Oocytes During the Latter Half of In Vitro Maturation and Pronuclear Formation Following Chemical Activation
- Autophagy Influences Maternal mRNA Degradation and Apoptosis in Porcine Parthenotes Developing In Vitro
- Interleukin-6 Enhances Porcine Parthenote Development In Vitro, through the IL-6/Stat3 Signaling Pathway
- Glycosaminoglycans Improves Early Development of Zona-free 8-cell Rat Embryos to Blastocysts in a Chemically Defined Medium, but Not the Pregnancy Rate Following Transfer of the Blastocysts
- Interleukin-6 Enhances Porcine Parthenote Development In Vitro, through the lL-6/Stat3 Signaling Pathway
- Autophagy Influences Maternal mRNA Degradation and Apoptosis in Porcine Parthenotes Developing In Vitro
- Knockdown of Maternal Homeobox Transcription Factor SEBOX Gene Impaired Early Embryonic Development in Porcine Parthenotes
- What is the optimal condition for fertilization of IVM oocytes?