In-vitro Culture with a Tilting Device in Chemically Defined Media During Meiotic Maturation and Early Development Improves the Quality of Blastocysts Derived from In-vitro Matured and Fertilized Porcine Oocytes

スポンサーリンク

概要

• 論文の詳細を見る

• Under physiological conditions, mammalian oocytes and embryos appear to be stimulated not only chemically but also mechanically, such as by compression, shear stress and/or friction force in the follicle and female reproductive tract. The present study was undertaken to examine the effects of kinetic culture with a tilting device in chemically defined media during in vitro maturation (IVM) of porcine oocytes and in vitro culture (IVC) following in vitro fertilization (IVF) on the early developmental competence and quality of blastocysts. After culture in a chemically defined IVM medium, modified porcine oocyte medium (mPOM) containing gonadotropins and dibutyryl cAMP for 20 h, the mean diameter of the cumulus-oocyte complexes (COCs) was larger in the tilting culture than in the static controls, whereas the diameter of the oocytes did not differ. When culture of the COCs was continued additionally in a fresh medium without gonadotropins and dibutyryl cAMP for 24 h, the incidences of oocytes completing GVBD and developing to the metaphase-II stage did not differ between the tilting and static culture systems. Furthermore, the sperm penetration after IVF and developmental competence of the oocytes to the blastocyst stage were not different between the tilting and static systems during IVM and IVC. However, tilting culture during both IVM and IVC had a significant positive effect on the number of cells per blastocyst (P<0.05). These observations indicate that tilting culture during IVM and IVC in chemically defined media improves the quality of blastocyst, as determined by the number of cells per blastocyst, without any effects on penetrability and developmental competence.

• 2010-10-01

著者

• MATSUURA Koji

  Research Core for Interdisciplinary Sciences, Okayama University

• FUNAHASHI Hiroaki

  Department of Animal Science, Graduate School of Natural Science and Technology, Okayama University

• Naruse Keiji

  Department Of Cardiovascular Physiology Graduate School Of Medicine Dentistry And Pharmaceutical Sci

• Matsuura Koji

  Research Core For Interdiciplinary Sciences Okayama University

• Funahashi Hiroaki

  Department Of Animal Science Graduate School Of Natural Science And Technology Okayama University

• Matsuura Koji

  Research Core For Interdisciplinary Sciences Okayama University

• Koike Takayuki

  Department Of Animal Science Graduate School Of Natural Science And Technology Okayama University

• Naruse Keiji

  Department Of Cardiovascular Physiology Graduate School Of Medicine Dentistry And Pharmaceutical Sci

• Funahashi Hiroaki

  Department Of Animal Science Faculty Of Agriculture Okayama University

• Matsuura Koji

  Res. Core For Interdisciplinary Sciences Okayama Univ.

関連論文

• In-vitro Culture with a Tilting Device in Chemically Defined Media During Meiotic Maturation and Early Development Improves the Quality of Blastocysts Derived from In-vitro Matured and Fertilized Porcine Oocytes
• Successful Piglet Production in a Chemically Defined System for In-vitro Production of Porcine Embryos : Dibutyryl Cyclic AMP and Epidermal Growth Factor-family Peptides Support In-vitro Maturation of Oocytes in the Absence of Gonadotropins
• 1TA5-06 マイクロ流体中の運動精子における細胞内カルシウムイオンが果たす一役割(細胞生物的課題(接着,運動,骨格,伝達,膜),第47回日本生物物理学会年会)
• PE-556 Impaired Coronary Capillary Flow with Decreased Glycocalyx Thickness and Irregular Inner Wall Remodeling in Right Ventricle of Pulmonary Hypertensive Rats(Coronary circulation, basic/clinical-3 (IHD) PE95,Poster Session (English),The 70th Anniversa
• 3P189 低分子量Gタンパク質Rac1の接着斑へのリクルート機構 : 1分子追跡法による解析(細胞生物的課題(接着,運動骨格,伝達,膜),第48回日本生物物理学会年会)
• FRS-041 Coronary Microvascular Endothelial Dysfunction with Decreased Glycocalyx Thickness and Increased Reactive Oxygen Species in Spontaneously Hypertensive Rats(Vascular Biology in Endothelial Cells (basic), The 71st Annual Scientific Meeting of the Ja
• Hydrophobic Silicone Elastomer Chamber for Recording Trajectories of Motile Porcine Sperms without Adsorption
• 特集 シリコン化学センサ
• Low Salt Maturation Medium Enhances the Histone H1 Kinase Activity of Porcine Oocytes at the End of In Vitro Maturation
• Hydrophobic Silicone Elastomer Chamber for Recording Trajectories of Motile Porcine Sperms without Adsorption
• In-vitro Culture with a Tilting Device in Chemically Defined Media During Meiotic Maturation and Early Development Improves the Quality of Blastocysts Derived from In-vitro Matured and Fertilized Porcine Oocytes
• Exogenous Adenosine Reduces the Mitochondrial Membrane Potential of Murine Oocytes During the Latter Half of In Vitro Maturation and Pronuclear Formation Following Chemical Activation
• 3L1334 タンパク質群島構造と分子拡散がミクロンサイズ接着班を促進する : 1分子法による研究(細胞生物的課題5,第49回年会講演予稿集)
• Wavelength-Scanning Method for Identifying Vertical Position of Film Using Surface Plasmon Microscopes
• Glycosaminoglycans Improves Early Development of Zona-free 8-cell Rat Embryos to Blastocysts in a Chemically Defined Medium, but Not the Pregnancy Rate Following Transfer of the Blastocysts
• 1PT211 マウス胚盤胞における細胞内カルシウム濃度とメカニカルストレス負荷の同時計測(日本生物物理学会第50回年会(2012年度))
• What is the optimal condition for fertilization of IVM oocytes?

もっと見る 閉じる

スポンサーリンク