A Newly Devised Assay Method for Administered Labeled Thiamine

概要

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The new simple technique for determination of administered labeled thiamine in biological materials was described.Outline of this method is based on the thiamine determination procedure of Fujiwara and Matsui. Tissue samples containing labeled thiamine were extracted with H2SO4, protein was precipitated and removed. The extract was adsorbed on Permutit column and labeled thiamine was eluted using hot KCI in 0.1 N HCI. To the eluate, cyanogen bromide in sodium hydroxide was added, the resulting labeled thiochrome was extracted into n-butanol.In case of determination using the gas flow counter the butanol layer was transferred into a counting dish, dried under an infrared lamp and counted. When determining by liquid scintillation the butanol was added directly to the counting solution in the vial.This method is simple, rapid and accurate. Moreover, it is possible to determine differentially free and esterified labeled thiamine.
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