Response of mononuclear cells cultured from giant cell tumor of bone to hyperthermia.

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A Cell line of mononuclear cells from giant cell tumor of bone was examined for its response to hyperthermic exposure. The cells were treated by water bath immersion while maintained in suspension culture in glass tubes. The suspension was gassed with 95% air plus 5% CO2 overnight prior to hyperthermic treatment. Hyperthermic treatment was carried out at temperatures of 43.5°C and 45.6°C, and survival cells were counted. Using a flow cytometry possessing rate of monocyte antigens of the cells and DNA pattern were analysed at both temperatures. The higher the temperature and the longer the time, the lesser the survival cells and the lesser the expression of the monocytic phenotype. None of the cells could be survived at a temperature of 45.6°C for 120min.
西日本整形・災害外科学会の論文