魚尿中のステロイドホルモン代謝産物-3-コイ尿中のコルチコステロイド代謝産物の検出と同定〔英文〕

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The present study deals with the identification of corticosteroid metabolites in the urine of carp, Cyprinus carpio. Samples of carp urinary steroids obtained by β-glucuronidase hydrolysis were converted to methoximetrimethylsilyl (MO-TMSi) derivatives and then subjected to gas liquid chromatographic (GLC) analysis. The MO derivatives were prepared by overnight reaction with methoxyamine hydrochloride; the TMSi derivatives were prepared by reaction with BSA (bis-trimethylsilylacetamide) at room temperature or TSIM (N-trimethylsilylimidazole) at IOO°C. The GLC separations were carried out on 1%OV-l and 2%QF-l columns. The retention data (MU values) of carp urinary steroids were compared with those of reference steroids identically treated with the above procedures. The results showed the presence of tetrahydrocortisone, tetrahydrocortisol, cortolone, β-cortolone, cortol, and β-cortol in the urine of carp. The structures of the former three com-pounds were also confirmed by GLC-Mass spectrometry.
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