コイ補体第2成分(C2)の血清からの単離〔英文〕

概要

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The second component (C2) of carp complement was isolated from carp serum by a six-step purification procedure: 1) affinity chromatography on Blue-Cellulofine, 2) affinity chromatography on heparin-agarose, 3) gel filtration on Sephadex G-200, 4) TSKgel DEAE-5PW chromatography (HPLC), 5) TSKgel Heparin-5PW chromatography (HPLC), and 6) TSKgel G3000SWXL chro-matography (HPLC). Hemolytic activity of carp C2 in column eluates was measured using EAC14 (sheep erythrocytes sensitized with carp antibody, C1 and C4) and heat-inactivated carp serum (deficient in C1 and C2) as reagents for detecting C2. About 0.4mg of carp C2 was obtained from 100ml of serum. The final recovery was 9.4%, representing a 799-fold purification. Carp C2 was composed of a single polypeptide chain with molecular weight of 108, 000 and was susceptible to heat-inactivation at 45°C. These physicochemi-cal properties of carp C2 closely resemble those reported for mammalian C2.
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