ペ-パ-・ディスクを用いる南極産オキアミのプロテア-ゼ簡易測定法

概要

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A convenient assay method for protease of the Antarctic Krill, Euphausia superba, which is well suited for measurements on a ship is proposed. A paper disk (diameter: 8mm) containing extracted juice of a Krill sample was put on the surface of a 10ml-casein-agar plate (1.5% agar 0.4% casein, 0.1M phosphate buffer, pH 7.0) and kept at 30°C for a definite time. The diameter (mm) of the clear halo which appeared after the treatment of the plate with 5% trichloroacetic acid was measured. In order to calculate the proteolytic activity (PU) of Krill samples, the solution of pancreatin of known activity was used as a reference standard. The relationship between the logarithm of the enzyme concentration and the diameter of the halo was definitely linear.
日本水産學會の論文