ラテックス凝集反応による軟体類および甲穀類の種の判別〔英文〕

概要

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Latex (Lx) agglutination tests have been employed in species indentification of molluscs and crustaceans. The tests were performed by slide glass method, and 0.5% suspension of polystyren Lx particles, were used for the tests. In general, antiserums of rats immunized with sarcoplasmic proteins of molluscs and crustanceans specifically agglutinated the Lx coated with homologous sarcoplasmic antigens, but some cross reactions were detected between the related species. To confirm the species specificity of reactions, the agglutination inhibition tests were made by adding the inhibiting antigen solution (muscle extract) into the agglutination reaction systems. The results showed that the identification of species was possible through these tests, when the suitable specific antiserum was used. Lx coated with antibody of rat immune serum was also specifically agglutinated by homologous antigen solution. It was concluded that the Lx agglutination test is very useful in identifying the species, since the reaction was very specific and much more sensitive in comparison with the micro-immunodif-fusion method. However, in the development of other studies using Lx agglutination, further investigation has to be carried out especially on the problems concerned with the unspecific agglutination occurring with muscle extracts of some teleost species.
日本水産學會の論文