サケ・タラ科魚類筋肉のグリセルアルデヒド-リン酸脱水素酵素のNAD含量とその添加効果〔魚類筋肉のグリセルアルデヒド-リン酸脱水素酵素に関する研究-2-〕

概要

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For the crystallization of purified glyceraldehyde-phosphate dehydrogenases (EC. 1.2.1.12) from fish muscles, the requirement of each enzyme for added NAD is different from species to species. Thus, we attempted to determine the bound NAD content in the enzymes. The results obtained by the titration with NAD and the ratio of E280:E260 indicated that each fish enzyme contained varying amounts of bound NAD and less than one mol of NAD per mol of enzyme. The exogenous NAD-added fish enzymes can be resolved into multiple forms having different amounts of NAD by ion exchange chromatography. The effect of NAD on the conformation of the enzyme was investigated by comaring the loss of enzyme activity in the presence of urea and the regain of enzyme activity on dilution of urea. In contrast to the rabbit enzyme, which reversed the high extent of its activity in the renaturation, the fish enzymes regained little or no activity. In the presence of NAD, the activities of fish enzymes were recoverable in both the denaturation and the renaturation. The fish enzymes supplied with added NAD attained to the same stability for urea as that of the rabbit enzyme.
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