サケ・タラ科魚類筋肉のグリセルアルデヒド-リン酸脱水素酵素の免疫学的性質〔魚類筋肉のグリセルアルデヒド-リン酸脱水素酵素に関する研究-3-〕

概要

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In order to undertake a more complete survey of the properties of the glyceraldehyde-phosphate dehudrogenase (EC. 1. 2. 1. 12) and to establish the taxonomic relationships among the species from which the eczyme were isolated, antiserum was prepared in rabbit against the enzyme purified from chum salmon muscle. Gel diffusion and enzyme inhibition experiments were carried out with the homologeneous enzymes isolated from other species of salmonid and gadoid fishes. The data obtained show the following results: (1) Each enzyme isolated from salmonoid fish showed a cross-reaction with the antiserum, indicating the immunological homology among these enzymes; (2) The antiserum cross-reacted weakly with the gadoid enzyme and its concentration was increased by 2-3 fold for the production of quantitative precipitin and enzyme inhibition in the cross-reacting system, indicating the partially structural similarity between the enzyme from the two bony fishes; (3) The antiserum did not cross-react with the rabbit enzyme over a wide concentration range, indicating considerable structural differences between the enzymes from the two organisms.
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