サケ・タラ科魚類筋肉のグリセルアルデヒド-リン酸脱水素酵素の精製〔魚類筋肉のグリセルアルデヒド-リン酸脱水素酵素に関する研究-1-〕

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Procedures are described for the preparation of crystalline glyceraldehydes-phosphate dehydrogenases (EC. 1. 2. 1. 12) from skeletal muscles of salmonid and gadoid fishes. The preparations were found to be homogeneous by the criteria of polyacrylamide gel electrophoresis and ultracentri-fugal analysis. The enzymes isolated from these sources can be readily crystallized in the presence of NAD, although there are some enzymes which crystallized without it. The subunit molecular weights determined by gel electrophoresis in the presence of sodium dodecyl sulfate and the sedimentation constants for each of these enzymes are approximately identical with those of rabbit muscle enzyme. This indicates that the fish enzymes are tetramic and are composed of four chemically identical polypeptide chains of molecular weight 36, 000.
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