アマノリ属の葉緑体DNAの制限酵素切断パタ-ンの種間差異〔英文〕

概要

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The rhodoplast DNA was isolated and purified from five species of Porphyra, three species widely cultivated in Japan, P. tenera, P. tenera f. tamatsuensis and P. yezoensis, and two naturally grown species, P. dentata and P. suborbiculata. Except P. yezoensis, plasmid-like DNA was found in multiple bands when undigested rhodoplast DNA was electrophoresed on agarose gels. The molecular sizes of these plasmids were different in the species examined. Upon digestion with the restriction endonuclease Xho I, the electrophoretic mobilities of most DNA fragments of the five species of algae were essentially similar, but some fragments characteristic in each species were also found. The present results suggest that the electrophoretic patterns of plasmid-like mole-cules and of the endonuclease digesting fragments may provide the useful tools for the species identification of Porphyra