海洋から分離したアミラ-ゼインヒビタ-生産菌培養条件の検討およびインヒビタ-amylostreptinの産生〔英文〕

概要

論文の詳細を見る
Streptomyces corchorusii subsp. rhodomarinus subsp. nov. which was isolated from marine sediment produced an extracellular α-amylase inhibitor named "amylostreptin". The optimal medium of the strain for the production of amylostreptin was investigated by using shake flask and an improved basal medium. Soluble starch as a carbon source and Polypepton as a nitrogen source were excellent for amylostreptin production as well as the growth of the strain. The maximum production of amylostreptin (62.6×103 units/0.1ml of broth) was observed in a medium consisting of 1.0% soluble starch, 1.2% Polypepton, and 0.1% Bacto-yeast extract at pH 7.5 in 25%(v/v) seawater. Under the optimal cultivation conditions mentioned above, the production of amylostreptin increased about 3 times compared to the initial level. The crude amylostreptin was very stable up to 100°C for 30 min as well as in a wide range of pH stability from 1 to 13.
日本水産學會の論文