海産プロテア-ゼインヒビタ-生産菌の分離とその分類学的諸性状〔英文〕

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Marine bacteria which produced extracellular alkaline protease inhibitor were isolated and identified. A new and simple casein double layer agar method was employed for a quick screening of the inhibitor producers. Out of the 878 isolates from Aburatsubo Inlet, Kanagawa, Japan, only three strains produced the inhibitor whereas no inhibitor producer was found out of the 1, 961isolates from other sea areas. Among the three inhibitor producers, strain B-10-31 showed the largest inhibitory zone, suggesting that this strain had the strongest inhibitory activity.All the inhibitor producing strains were aerobic, polarly flagellated, Gram-negative, rodshaped organisms with low G+C contents in their DNAs (39.9 to 42.0mol%). They required NaCI for growth, which indicates that they are of marine origin. All the strains hydrolyzed complex polymers like casein, DNA, gelatin and starch. None of them utilized organic acids. Based on these taxonomical characteristics, they were identified as Alteromonas spp. Although the three strains belonged to the same genus, strain B-10-31 differed considerably in its characteristics from the other two (B-3-62 and B-3-87).This is the first report of a protease inhibitor producing bacteria isolated from seawater.
社団法人日本水産学会の論文