マサバ幽門垂および腸のジアミンオキシダ-ゼについて

概要

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The homogenates of the pyloric caeca and the intestine of common mackerel Scomber japonicus were fractionated by DEAE-cellulose column chromatography. The fractions exhibiting diamine oxidase activity were searched and some properties of the enzymes were examined. Three protein fractions were observed in both the chromatogram, and the diamine oxidase activities which were assayed by using histamine and cadaverine as the substrates which were recognized among the two. The follwing properties were identical in the two fractions from both the organs: The optimal pH values of the enzymes were at 7.3 and 8.0 when histamine and cadaverine were utilized as the substrates respectively. From the examination on the substrate specificity it was observed that the activities on histamine and cadaverien were higher than those on agmatine, and the highest activity was shown on cadaverine. The activities were inhibited slightly by EDTA and strongly by aminoguanidine. The properties of the diamine oxidase in the pyloric caeca and the intestine were indentical, but different from those of the liver enzyme in the fractionation patterns, optimal pH values and substrate specificities.
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