再生セルロース中空糸 (BMM) を用いたヒト免疫不全ウイルス (HIV) 除去機構

概要

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We intended to show filtration characteristics of BMM for solutions containing HIV and to clarify membrane separation mechanism for HIV particles and to demonstrate an ideal membrane for HIV removal. The hollow fibers with mean pore sizes (2 γf) ranging from 10 nm to 105 nm were prepared from the cuprammonium solution of cellulose through the micro-phase separation method. The particle of HIV was obtained from cell-free culture supernatant of MOLT-4/HIV cells. Pore characteristics and distribution of HIV in BMM were evaluated through electronmicroscopy. The concentration of HIV was evaluated through the plaque forming method. Filtration experiments were carried out at transmembrane pressures ranging from 20 mmHg to 1000 mmHg and filtration volumes of less than 55 l/m2. All filtrate didnt have HIV in detectable level. The electronmicroscopy revealed that BMM had a multi-layer structure and HIV was caught in BMM by the multi-step filtration mechanism. The pore size distribution of each layer can be designed so as to get high recovery of protein during HIV free filtration.