Pyridoxal 5-Phosphate Modulates Expression of Cytosolic Aspartate Aminotransferase Gene by Inactivation of Glucocorticoid Receptor.
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概要
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The level of mRNA for cytosolic aspartate aminotransfer-ase (cAST) in the liver of vitamin B6-deficient rats was found to be 7-fold higher than that of the control rats. The administration of hydrocortisone to adrenalectomized vitamin B6-deficient rats induced expression of hepat-ic cAST mRNA and the induction was suppressed by the simultaneous administration of pyridoxine. Since the 5' regulatory region of the rat cAST gene contains several sequences showing homology to glucocorti-coid-responsive elements, we synthesized an oligonucleotide probe of glucocorticoid-responsive element sequence and assayed the binding activ-ity of liver nuclear extract to the oligonucleotide by gel mobility shift analysis. We found that the binding activity of nuclear extract prepared from the liver of vitamin B6-deficient rats was far greater than that of the control rats, indicating that the DNA-binding activity of glucocorticoid receptor was enhanced by vitamin B6 deficiency. We further found that preincubation of the nuclear extract from the vitamin-deficient liver with pyridoxal 5'-phosphate brought about a rapid and extensive decrease in the binding of the extract to the glucocorticoid-responsive element. Con-geners of pyridoxal phosphate, such as pyridoxamine 5'-phosphate, pyri-doxal, pyridoxamine and pyridoxine, did not show an inhibitory effect. These observations suggest that pyridoxal 5'-phosphate modulates cAST gene expression by inactivating the binding activity of glucocorticoid receptor to glucocorticoid-responsive elements.
- 財団法人 学会誌刊行センターの論文
著者
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Oka Tatsuzo
Department Of Nutrition School Of Medicine The University Of Tokushima
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Natori Yasuo
Department Of Nutrition School Of Medicine The University Of Tokushima
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KUWAHATA Masashi
Department of Molecular Nutrition, Institute of Health Biosciences, The University of Tokushima Grad
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HIROI Yuzo
Department of Food and Nutrition, Nakamura Gakuen College
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KOMORI Naomi
Department of Nutritional Chemistry, School of Medicine, The University of Tokushima
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SHIMOHA Taeko
Faculty of Home Economics, Kyushu Women's University
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OKAHA Mitsuko
Faculty of Health and Living Science, Naruto University of Education
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SHIMOHA Taeko
Faculty of Home Economics, Kyushu Women's University
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OKA Tatsuzo
Department of Nutritional Chemistry, School of Medicine, The University of Tokushima
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OKA Tatsuzo
Department of Frontier Veterinary Science, Faculty of Agriculture, Kagoshima University
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- Pyridoxal 5-Phosphate Modulates Expression of Cytosolic Aspartate Aminotransferase Gene by Inactivation of Glucocorticoid Receptor.