Effect of Pyridoxine-Deficiency on Degradation of Cytosolic Aspartate Aminotransferase in Rat Liver Lysosomes.
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概要
- 論文の詳細を見る
Highly purified lysosomes were isolated from the livers of control and pyridoxine-deficient rats. The calculation of the lysosomal protein contents indicated that the livers of both groups of rats contain virtually the same amounts of the lysosomal proteins (12.0 and 13.0mg lysosomal proteins/g liver proteins for the control and pyridoxine-deficient rats, respectively). The immunoblotting of the lysosomal proteins with anti-cytosolic aspartate aminotransferase (cAspAT) showed 46 kDa band, corresponding to the subunit molecular weight of cAspAT, as well as the bands representing degradative intermediates of cAspAT. The relative amounts of the immuno-reactive substances were estimated by scanning the immuno-stained bands and measuring the densitometric tracings. It was found that the lysosomes in the pyridoxine-deficient rat liver contain almost twice as much cAspAT and its degradative intermediates as those in the control rat liver. On the basis of these observations, it was concluded that the increased rate of degradation of cAspAT in the liver of the pyridoxine-deficient rats is brought about by the increased rate of sequestration of cAspAT into lysosomes.
- 財団法人 学会誌刊行センターの論文
著者
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Natori Yasuo
Department Of Nutrition School Of Medicine The University Of Tokushima
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Sato Atsushi
Department Of Applied Chemistry Chubu University
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OKADA MITSUKO
Department of Enzyme Chemistry, Institute for Enzyme Research, School of Medicine, Tokushima University, Tokushima and Department of Metabolism, Institute for Protein Resarch, Osaka University
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NAGAI Mizue
Department of Nutritional Chemistry, School of Medicine, The University of Tokushima
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TAGAMI Ayuri
Department of Nutritional Chemistry, School of Medicine, The University of Tokushima
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OKADA Mitsuko
Department of Home Economics, Faculty of Health and Living Sciences, Naruto University of Education
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