DIFFERENCES BETWEEN THE TRANSAMINASES IN MITOCHONDRIA AND SOLUBLE FRACTION:I. GLUTAMIC-PYRUVIC TRANSAMINASE
スポンサーリンク
概要
- 論文の詳細を見る
1. Sonication, deoxycholate treatment, freezing and thawing and mechanical grinding techniques were tried to solubilize the glutamic-pyruvic transaminase (GPT) from mitochondria and the enzymes having the same activities were obtained.2. Glutamic-pyruvic transaminases in soluble fraction and mitochondria (GPTS and GPTM) were eluted with 0.02M phosphate buffer, pH 6.8, and 0.06M phosphate buffer, pH 6.8, respectively, in calcium phosphate gel column chromatography by stepwise and gradient eluting systems. In zone electrophoresis, they moved to the cathode and could not be separated from each other. These enzymes also have quite different optimal pH values.3. Michaelis constants of GPT and their elementary reactions were determined and found to be almost equal in both crude and purified GPTS and GPTM reactions.
- 日本ビタミン学会の論文
著者
-
OKADA MITSUKO
Department of Enzyme Chemistry, Institute for Enzyme Research, School of Medicine, Tokushima University, Tokushima and Department of Metabolism, Institute for Protein Resarch, Osaka University
-
MATSUDA MAKOTO
Department of Biochemistry, Jikei-University School of Medicine
-
KATUNUMA NOBUHIKO
Department of Biochemistry, Faculty of Medicine, Nagoya University
-
MIKUMO KAZUKO
Department of Metabolism, Institute for Protein Research, Osaka University, Osaka
関連論文
- In-beam γ-ray spectroscopy of neutron-rich ^Pu and properties of N=152 deformed shell closure(International Workshop on Nuclear Structure-New Pictures in the Extended Isospin Space(NS07)-)
- Ground-State Band of the Neutron-Rich Transuranium Nucleus ^Cm_(Nuclear Physics)
- Cerebral Acetylcholine Levels and Long-Term Spatial Cognitive Impairment Following Middle Cerebral Artery Occlusion in Rats
- EFFECT OF PYRIDOXAL PHOSPHATE DEFICIENCY ON AROMATIC L-AMINO ACID DECARBOXYLASE ACTIVITY WITH L-DOPA AND L-5-HYDROXYTRYPTOPHAN AS SUBSTRATES IN RATS
- ENZYMATIC STUDY ON FOLIC ACID BIOSYNTHESIS BY MYCOBACTERIUM AVIUM
- DIFFERENCES BETWEEN THE TRANSAMINASES IN MITOCHONDRIA AND SOLUBLE FRACTION:II. GLUTAMIC-OXALOACETIC TRANSAMINASE
- Effect of Pyridoxine-Deficiency on Degradation of Cytosolic Aspartate Aminotransferase in Rat Liver Lysosomes.
- DIFFERENCES BETWEEN THE TRANSAMINASES IN MITOCHONDRIA AND SOLUBLE FRACTION:I. GLUTAMIC-PYRUVIC TRANSAMINASE
- Absorption and Metabolism of Pyridoxamine in Mice. I.Pyridoxal as the Only Form of Transport in Blood.
- A SIMPLIFIED PREPARATION METHOD OF P32- LABELED FLAVIN MONONUCLEOTIDE AND FLAVIN-ADENINE DINUCLEOTIDE AND THEIR APPLICATION TO BIOCHEMICAL RESEARCHES
- Chemical Structure and Biological Function of Amplifying Factors of Glucocorticoid Actions
- Effect of Ammonia on Tricarboxylate Utilization in Rat Liver Mitochondria