A SIMPLIFIED PREPARATION METHOD OF P32- LABELED FLAVIN MONONUCLEOTIDE AND FLAVIN-ADENINE DINUCLEOTIDE AND THEIR APPLICATION TO BIOCHEMICAL RESEARCHES
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概要
- 論文の詳細を見る
A simple preparation method of P32-labeled FAD following injection of P32-phosphate and FMN in rabbits was investigated. Hydrolysis of P32-FAD thus obtained results in the production of P32-FMN. In this case the phosphor of FMN has probably been exchanged for P32. It can be understood by the reversibility of the reaction: Riboflavin+PO43-⇔FMN. Although the ratio of incorporation of P32 into FAD is very low, the radioactivity of the P32-FAD thus obtained is strong enough to be used for biochemical experiments. The P32-FMN obtained from P32-FAD was utilized for investigation of riboflavin metabolism in vivo and the mechanism of riboflavin photolysis.
- 日本ビタミン学会の論文
著者
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Hotta Kazuo
Department Of Pharmacy And Pharmacokinetics Aichi Medical University School Of Medicine
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Ishiguro Isao
Department Of Biochemistry School Of Medicine Fujita Health University
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Sugiura Mamoru
Department Of Pharmacy Gifu College Of Pharmacy
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KATUNUMA NOBUHIKO
Department of Biochemistry, Faculty of Medicine, Nagoya University
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KOJIMA KAZU
Department of Ophthalmology, Faculty of Medicine, Nagoya University
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HISHIKI TOSHIYUKI
Department of Ophthalmology, Faculty of Medicine, Nagoya University
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IKEGAMI YOSHIO
Department of Ophthalmology, Faculty of Medicine, Nagoya University
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HOTTA KAZUO
Department of Biochemistry, Faculty of Medicine, Nagoya University
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