ヒト顎関節関節円板の細胞外基質の免疫組織化学的研究 : 第1報: ヒト正常顎関節関節円板について

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Although many investigations for pathogenesis of temporomandibular joint (TMJ) disorders have been made by biochemical and histochemical methods, it has not been elucidated completely. In the present study, a preliminary examination was made concerning localization on some of proteoglycans (PGs), glycosaminoglycans (GAGs) and tenascin a glycoprotein in the articular disc of human adult TMJ immunohistochemically.The fresh materials of the normal articular discs were obtained from 3 adult cadavers within 48 hours of mortality.The 9A2, 3B3, 1B5, CS56, 6B6, 2B1, 5D4 and anti-tenascin antibody were utilized as primary antibodies by the indirect immunohistochemical method. As a result, we found the following three points. Firstly, chondroitin 6-sulfate and keratin sulfate were localized intensively in the articular disc, whereas, chondroitin 4-sulfate could not be detected in the disc. Secondly, small DS-PGI were recognized intensively not only in the articular disc but also in the retrodiscal fat pad, which were stronger than small DS-PG II. Thirdly, large PG could not be observed except on the vessel wall of capillaries in the retrodiscal fat pad.tenascin was identified at perineurium and some parts of synovial membran on the inferior cavity of TMJ. We concluded that the toughness and flexibility of the articular disc against loading might be attributable to the above-mentioned structure of the reasonably composed extracellular matrix (ECM).