女王蜂幼虫の日齢に伴うローヤルゼリーの窒素成分組成の消長

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Chemical components of royal jelly (RJ) were investigated from larvae of different ages. The moisture and sugar contents were lowest in RJ collected from the youngest cell (24 hr after grafting) and increased in the 48 hr samples, then decreased thereafter. The crude protein content and the titratable acidity of the samples showed a Ushaped change, in which the highest amount was observed in RJ obtained from the youngest larvae. The amount of soluble and protein nitrogen and free amino acids were found to be constant throughout the examined periods. The soluble protein of the RJ obtained from youngest larvae was separated by Sephadex G-100 chromatography and by polyacrylamide gel electrophoresis. The chromatogra-phic and electrophoretic patterns of the protein fractions of the different RJ were similar.