アカマツ花粉ミトコンドリアの酵素〔花粉の生化学的研究-17-〕

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Subcellular fractions were prepared from the pollen of Pinus densiflora, and the mitochondrial fraction was assayed for isocitrate dehydrogenase, succinate dehydrogenase and cytochrome oxidase activities. The isocitrate dehydrogenase required NADP for its reaction, while the activity with NAD was about 8% of that with NADP. DNA-P was mainly localized in the nuclear fraction, and succinate dehydrogenase and cytochrome oxidase in the fraction sedimented at 15, 000×g for 20min. The particulate fraction sedimented at 15, 000×g was presumed to be the mitochondrial fraction from the staining with Janus Green B, their Brown's movement, electron microscopic appearance and enzyme activities. Both dehydrogenases and cytochrome oxidase activities in the mitochondrial fraction increased during germination of the pollen on sucrose-agar medium.
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