幽門垂蛋白分解酵素に関する研究-2-

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After purification of the proteinase of tunny pyloric caeca with Amberlite XE-64 (Fig. 1-5) and carrying out of the column chromatography with Amberlite IRC-50 (Fig. 6) as in the preliminary experiments, the preparation of crystalline proteinase was made as follows: distilled water extraction of ground pyloric caeca, adsorption of enzyme with Amberlite XE-64, elution of proteinase after washing the resin for removal of amylase, salting-out with ammonium sulphate, dialysis, precipitation and crystallization with acetone (Fig. 7). Crystals of the proteinase showed the same form as in the previous report. The crystalline proteinase was obtained with an yield of 4.8% in activity, and a specific activity increased 20.9 times as compared with the original solution (Table 2).
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