幽門垂蛋白分解酵素に関する研究-1-

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The purification of the proteinase of tunny pyloric caeca was carried out as follows; the distilled water extraction of ground material, salting-out with ammonium sulphate, dialysis, rivanol treatment for removal of amylase, fuller's earth treatment, and precipitation and cry-stallization with acetone (Fig. 3). The crystals of proteinase were obtained in the form of needles (Fig. 4). The crystalline proteinase was obtained with an yield of 1.5% in activity and a specific activity increased 20.6 times as compared with the original solution (Table 3).
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