幽門垂蛋白分解酵素に関する研究-3-

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The purification of the proteinase of bonito pyloric caeca was carried out as follows; distilled water extraction of ground pyloric caeca, adsorption of enzyme with Amberlite XE-64, elution of proteinase after washing the resin for removal of amhlase, salting-out with ammonium sulphate, dialysis, decolorization with Duolite A-2, precipitation and crystallization with acetone (Fig. 1). Crystals of the proteinase were obtained in the form of needles (Fig. 2). The crystalline proteinase was obtained with an yield of 2% in activity and a specific activity increased 24 times as compared with the original solution (Table 3).
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