Metabolism of sulfobromophthalein in jaundiced rat.

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We observed previously in humans that loading with BSP in obstructive jaundice resulted in elevated blood levels, of cysteine-conjugated BSP (Cyst-BSP).In order to clarify the mechanism of this phenomenon, an investigation was made into the origin of Cyst-BSP in rats with experimentally produced obstruction of bile duct (O-J).Using untreated rats as controls, the animals were determined for glutathione content and glutathione S transferase activity (GST) in liver cytosol.The influence of nephrectomy upon and the role of liver cell menbrane in biodisposition of BSP were also investigated.The results are summarized as follows:1) In the O-J group, the excretion in bile of glutathione-conjugated BSP (GSH-BSP) was markedly decreased.2) O-J livers were found to have higher glutathione content and GST activity as compared to control livers.3) Nephrectomy in O-J rats was followed by no gross change in the proportion of Cyst-BSP in blood and liver cytosol.4) Following administration of individual liver cell membrane components change from GSH- BSP to Cyst-BSP was noted to occur only in O-J rats.These results led us to surmise that GSH-BSP accumulated in the liver in O-J is hydrolyzed to Cyst-BSP in the liver cell membrane.
財団法人日本消化器病学会の論文