A STUDY ON IDENTIFICATION OF GASTRIN CELL WITH INDIRECT IMMUNOPEROXIDASE METHOD

概要

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On identifying gastrin cell using indirect immunoperoxidase method, authors analyzed the process of tissue specimens before the immunological procedures, and investigated the merits of this method comparing with immunofluorescence.Sections stained with indirect immunoperoxidase method can be observed with conventional microscope, and furthermore, adding the poststaining to them, glandular structure in the back-ground becomes much clear, and the autofluorescent cells can be excluded. From these points of view, it can be said that the indirect immunoperoxidase method is more suitable for identifying gastrin cells than the immunofluorescence.As to the treatment of tissue specimens, the conventional techniques, i.e. fixation with formalin and embedding into paraffin, is available for obtaining the specific stain to gastrin cells, and this specific stainability of gastrin cell is sufficiently preserved even in a section which was stained with hematoxylin and eosin one year before.It can be emphasized that these procedures are convenient and suitable for histological investigations.
財団法人日本消化器病学会の論文